Expression of a modified Cry1Ie gene in E. coli and in transgenic tobacco confers resistance to corn borer.

作者信息

Liu Yun-Jun, Song Fu-Ping, He Kang-Lai, Yuan Yuan, Zhang Xiao-Xia, Gao Peng, Wang Jian-Hua, Wang Guo-Ying

机构信息

State Key Laboratory for Agrobiotechnology, National Center for Maize Improvement, China Agricultural University, Beijing.

出版信息

Acta Biochim Biophys Sin (Shanghai). 2004 Apr;36(4):309-13. doi: 10.1093/abbs/36.4.309.

DOI:10.1093/abbs/36.4.309

PMID:15253158

Abstract

The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.

摘要