Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Sci Rep. 2017 Jan 31;7:41688. doi: 10.1038/srep41688.
Cry1Ie protein derived from Bacillus thuringiensis (Bt) has been proposed as a promising candidate for the development of a new Bt-maize variety to control maize pests in China. We studied the response of the midgut bacterial community of Apis cerana cerana to Cry1Ie toxin under laboratory conditions. Newly emerged bees were fed one of the following treatments for 15 and 30 days: three concentrations of Cry1Ie toxin (20 ng/mL, 200 ng/mL, and 20 μg/mL) in sugar syrup, pure sugar syrup as a negative control and 48 ng/mL imidacloprid as a positive control. The relative abundance of 16S rRNA genes was measured by Quantitative Polymerase Chain Reaction and no apparent differences were found among treatments for any of these counts at any time point. Furthermore, the midgut bacterial structure and compositions were determined using high-throughput sequencing targeting the V3-V4 regions of the 16S rDNA. All core honey bee intestinal bacterial genera such as Lactobacillus, Bifidobacterium, Snodgrassella, and Gilliamella were detected, and no significant changes were found in the species diversity and richness for any bacterial taxa among treatments at different time points. These results suggest that Cry1Ie toxin may not affect gut bacterial communities of Chinese honey bees.
Cry1Ie 蛋白来源于苏云金芽孢杆菌（Bt），被提议作为开发新的 Bt-玉米品种的有前途的候选物，以控制中国玉米害虫。我们研究了中肠细菌群落对 Cry1Ie 毒素的反应中华蜜蜂在实验室条件下。新出现的蜜蜂被喂食以下处理之一，持续 15 天和 30 天：Cry1Ie 毒素（20ng/ml、200ng/ml 和 20μg/ml）在糖水中的三个浓度、纯糖水作为阴性对照和 48ng/ml 吡虫啉作为阳性对照。16S rRNA 基因的相对丰度通过定量聚合酶链反应进行测量，在任何时间点，任何处理都没有发现这些计数之间存在明显差异。此外，使用针对 16S rDNA 的 V3-V4 区域的高通量测序来确定中肠细菌结构和组成。所有核心蜜蜂肠道细菌属，如乳杆菌属、双歧杆菌属、Snodgrassella 属和 Gilliamella 属都被检测到，并且在不同时间点的任何处理中，任何细菌类群的物种多样性和丰富度都没有发现明显变化。这些结果表明，Cry1Ie 毒素可能不会影响中国蜜蜂的肠道细菌群落。
