在牛肾上腺皮质中检测到一种脂质物质，该物质经碱处理后可产生孕烯醇酮。
Detection in bovine adrenal cortex of a lipoidal substance that yields pregnenolone upon treatment with alkali.
作者信息
Hochberg R B, Bandy L, Ponticorvo L, Lieberman S
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):941-5. doi: 10.1073/pnas.74.3.941.
Bovine adrenal cortical tissue contains a lipoidal derivative of pregnenolone (3beta-hydroxy-pregn-5-en-20-one) from which the free steroid can be liberated by treatment with alkali. Evidence for the presence of such an entity comes from examination of a nonpolar extract of tissue from which pregnenolone and its sulfate had been removed by chromatography. Treatment of the nonpolar fraction with alkali followed by exhaustive chromatographic analysis led to the detection of pregnenolone. The steroid was identified by both gas chromatography/mass spectrometry and double isotope procedures. Quantitative analysis indicated that the three forms of pregnenolone are present in bovine adrenal cortical tissue in the following amounts (mug/kg): lipoidal derivative, 290; free steroid, 435; and sulfate, 65. Because the only known metabolic function of pregnenolone is to serve as a precursor of the steroid hormones, these findings have far-reaching implications for steroid hormone biochemistry.
牛肾上腺皮质组织含有孕烯醇酮（3β-羟基-孕-5-烯-20-酮）的一种脂类衍生物，通过用碱处理可从中释放出游离类固醇。这种物质存在的证据来自对经色谱法去除了孕烯醇酮及其硫酸盐的组织非极性提取物的检测。用碱处理非极性部分，然后进行详尽的色谱分析，从而检测到了孕烯醇酮。该类固醇通过气相色谱/质谱法和双同位素程序进行了鉴定。定量分析表明，孕烯醇酮的三种形式在牛肾上腺皮质组织中的含量如下（微克/千克）：脂类衍生物，290；游离类固醇，435；硫酸盐，65。由于孕烯醇酮唯一已知的代谢功能是作为类固醇激素的前体，这些发现对类固醇激素生物化学具有深远的影响。
Zotero 插件