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医院水系统中军团菌的分布:免疫和基因相关的嗜肺军团菌血清群6分离株的流行情况

Distribution of legionellae in a hospital water system: prevalence of immunologically and genetically related Legionella pneumophila serogroup 6 isolates.

作者信息

Ott M, Bender L, Lück P C, Meyer P, Hacker J

机构信息

Lehrstuhl für Mikrobiologie, Universität Würzburg, FRG.

出版信息

FEMS Microbiol Lett. 1992 Aug 15;74(2-3):201-5. doi: 10.1016/0378-1097(92)90429-r.

Abstract

A hospital warm water system was monitored for the presence and distribution of legionellae. Subtyping of ten selected Legionella pneumophila isolates, originating from four different sites in the system by using serogroup specific antisera in an indirect immunofluorescence test, revealed that nine of the ten isolates belong to serogroup 6, while the remaining one was serogroup 10. Two monoclonal antibodies (mAbs) specific for a subgroup of serogroup 6 strains were further used for characterization. None of the strains reacted with these mAbs. Genome analysis by elaborating NotI profiles using the pulsed field gel electrophoresis (PFGE) technique revealed that nearly all serogroup 6 isolates derived from different sites, including a new building connected by a ring pipe, were identical according to restriction fragment patterns. The patterns were distinguishable from those of the two L. pneumophila serogroup 6 reference strains, and from that of the L. pneumophila serogroup 10 isolate. These data argue for a relatively homogeneous L. pneumophila serogroup 6 population in the entire water system.

摘要

对一家医院的温水系统进行了军团菌存在情况及分布的监测。通过间接免疫荧光试验,使用血清群特异性抗血清对从该系统四个不同位置选取的10株嗜肺军团菌分离株进行分型,结果显示10株分离株中有9株属于血清群6,其余1株属于血清群10。进一步使用两种针对血清群6菌株一个亚群的单克隆抗体(mAbs)进行特性鉴定。没有菌株与这些单克隆抗体发生反应。采用脉冲场凝胶电泳(PFGE)技术构建NotI酶切图谱进行基因组分析,结果显示,几乎所有来自不同位置(包括通过环形管道连接的一座新建筑)的血清群6分离株,根据限制性片段模式都是相同的。这些模式与两株嗜肺军团菌血清群6参考菌株的模式以及嗜肺军团菌血清群10分离株的模式不同。这些数据表明整个水系统中嗜肺军团菌血清群6的群体相对同质。

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