通过宏观限制性分析检测嗜肺军团菌血清群3和4菌株中的DNA多态性及其在医院内军团菌病流行病学调查中的应用。
DNA polymorphisms in strains of Legionella pneumophila serogroups 3 and 4 detected by macrorestriction analysis and their use for epidemiological investigation of nosocomial legionellosis.
作者信息
Lück P C, Köhler J, Maiwald M, Helbig J H
机构信息
Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität Dresden, Germany.
出版信息
Appl Environ Microbiol. 1995 May;61(5):2000-3. doi: 10.1128/aem.61.5.2000-2003.1995.
Abstract
Genomic DNAs of clinical and environmental isolates of Legionella pneumophila belonging to serogroups 3 and 4 were analyzed by macrorestriction analysis by pulsed-field gel electrophoresis. The restriction enzymes SfiI and NotI allowed easy visual separation of epidemiologically unrelated serogroup 3 strains. Three unrelated serogroup 3 strains that were isolated from different locations were identical by this genome mapping technique. Five unrelated serogroup 4 strains were separable by this technique. The electrophoretic patterns obtained after SfiI or NotI cleavage of the DNA of strains isolated from four patients with hospital-acquired legionellosis were identical to the patterns of strains isolated from the hot water supply systems of the buildings in which the patients were hospitalized. In conclusion, macrorestriction analysis is a valuable tool for epidemiological studies of infections caused by L. pneumophila serogroups 3 and 4.
摘要
采用脉冲场凝胶电泳进行宏观限制性分析，对嗜肺军团菌血清群3和4的临床及环境分离株的基因组DNA进行了分析。限制性内切酶SfiI和NotI能使流行病学上不相关的血清群3菌株易于通过视觉区分开来。通过这种基因组图谱技术，从不同地点分离出的3株不相关血清群3菌株是相同的。5株不相关血清群4菌株可通过该技术区分开来。对4例医院获得性军团病患者分离菌株的DNA经SfiI或NotI酶切后得到的电泳图谱，与从患者住院所在建筑物的热水供应系统中分离出的菌株图谱相同。总之，宏观限制性分析是对嗜肺军团菌血清群3和4引起的感染进行流行病学研究的宝贵工具。
相似文献
1
DNA polymorphisms in strains of Legionella pneumophila serogroups 3 and 4 detected by macrorestriction analysis and their use for epidemiological investigation of nosocomial legionellosis.通过宏观限制性分析检测嗜肺军团菌血清群3和4菌株中的DNA多态性及其在医院内军团菌病流行病学调查中的应用。
Appl Environ Microbiol. 1995 May;61(5):2000-3. doi: 10.1128/aem.61.5.2000-2003.1995.
2
Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak.使用脉冲场凝胶电泳进行核糖体分型和限制性酶切分析以区分医院感染暴发期间获得的嗜肺军团菌分离株的比较
J Clin Microbiol. 1992 Jun;30(6):1491-8. doi: 10.1128/jcm.30.6.1491-1498.1992.
3
Field inversion gel electrophoretic analysis of Legionella pneumophila strains associated with nosocomial legionellosis in children.与儿童医院获得性军团菌病相关的嗜肺军团菌菌株的场反转凝胶电泳分析。
J Clin Microbiol. 1996 Jan;34(1):175-6. doi: 10.1128/jcm.34.1.175-176.1996.
4
[Molecular comparison of Legionella pneumophila serogroup 1 isolated in Tunisia].[突尼斯分离的嗜肺军团菌血清1型的分子比较]
Pathol Biol (Paris). 2008 Jul;56(5):279-82. doi: 10.1016/j.patbio.2007.08.012. Epub 2008 Jan 10.
5
Outbreak of Legionnaire's Disease Caused by Legionella pneumophila Serogroups 1 and 13.由嗜肺军团菌血清群1和13引起的军团病暴发
Emerg Infect Dis. 2017 Feb;23(2):349-351. doi: 10.3201/eid2302.161012.
6
Pulsed field electrophoresis of genomic restriction fragments for the detection of nosocomial Legionella pneumophila in hospital water supplies.用于检测医院供水系统中院内嗜肺军团菌的基因组限制性片段脉冲场电泳
J Clin Microbiol. 1991 Apr;29(4):813-5. doi: 10.1128/jcm.29.4.813-815.1991.
7
Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis.通过任意引物PCR和脉冲场凝胶电泳对来自患者和医院环境的嗜肺军团菌血清1型分离株进行分子分型。
J Med Microbiol. 1999 Apr;48(4):327-333. doi: 10.1099/00222615-48-4-327.
8
A hospital-associated outbreak of Legionnaires' disease caused by Legionella pneumophila serogroups 4 and 10 with a common genetic fingerprinting pattern.一起由嗜肺军团菌血清型4和10引起的、具有共同基因指纹图谱模式的医院相关军团病暴发。
APMIS. 2004 Mar;112(3):210-7. doi: 10.1111/j.1600-0463.2004.apm1120307.x.
9
Genetic diversity of Legionella pneumophila in hospital water systems.医院供水系统中嗜肺军团菌的遗传多样性
Int J Hyg Environ Health. 2008 Mar;211(1-2):172-8. doi: 10.1016/j.ijheh.2007.04.003. Epub 2007 Jul 24.
10
Detecting legionellosis by unselected culture of respiratory tract secretions and developing links to hospital water strains.通过对呼吸道分泌物进行非选择性培养来检测军团菌病，并建立与医院水源菌株的联系。
J Hosp Infect. 1999 Apr;41(4):301-11. doi: 10.1053/jhin.1998.0517.
引用本文的文献
1
Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates.用于对比比利时嗜肺军团菌疫情分离株分型的全基因组测序分型工具
Eur J Clin Microbiol Infect Dis. 2025 Mar;44(3):597-607. doi: 10.1007/s10096-024-05013-4. Epub 2024 Dec 21.
2
Molecular Typing of Legionella pneumophila Isolates in the Province of Quebec from 2005 to 2015.2005年至2015年魁北克省嗜肺军团菌分离株的分子分型
PLoS One. 2016 Oct 5;11(10):e0163818. doi: 10.1371/journal.pone.0163818. eCollection 2016.
3
Nosocomial pneumonia caused by three genetically different strains of Legionella pneumophila and detection of these strains in the hospital water supply.由三种基因不同的嗜肺军团菌菌株引起的医院获得性肺炎以及在医院供水系统中对这些菌株的检测。
J Clin Microbiol. 1998 Apr;36(4):1160-3. doi: 10.1128/JCM.36.4.1160-1163.1998.
4
Uses of inorganic hypochlorite (bleach) in health-care facilities.无机次氯酸盐（漂白剂）在医疗保健机构中的用途。
Clin Microbiol Rev. 1997 Oct;10(4):597-610. doi: 10.1128/CMR.10.4.597.
本文引用的文献
1
Prevalence of Legionella species, serogroups, and monoclonal subgroups in hot water systems in south-eastern Germany.德国东南部热水系统中军团菌属、血清群和单克隆亚群的流行情况。
Zentralbl Hyg Umweltmed. 1993 Feb;193(5):450-60.
2
Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.嗜肺军团菌血清1型分型的任意引物聚合酶链反应、核糖体分型和单克隆抗体分析比较
J Clin Microbiol. 1993 Jul;31(7):1940-2. doi: 10.1128/jcm.31.7.1940-1942.1993.
3
Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting.通过聚合酶链反应介导的DNA指纹图谱对嗜肺军团菌菌株进行分型。
J Clin Microbiol. 1993 Aug;31(8):2198-200. doi: 10.1128/jcm.31.8.2198-2200.1993.
4
Outbreak of Legionnaire's disease linked to a decorative fountain by molecular epidemiology.通过分子流行病学将军团病暴发与一个装饰性喷泉联系起来。
Am J Epidemiol. 1993 Oct 15;138(8):555-62. doi: 10.1093/oxfordjournals.aje.a116895.
5
Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10.通过宏观限制性分析和使用单克隆抗体对嗜肺军团菌血清群10引起的医院获得性肺炎进行的流行病学调查。
J Clin Microbiol. 1994 Nov;32(11):2692-7. doi: 10.1128/jcm.32.11.2692-2697.1994.
6
Enhancement of recovery of Legionella pneumophila from contaminated respiratory tract specimens by heat.通过加热提高从受污染呼吸道标本中回收嗜肺军团菌的效率。
J Clin Microbiol. 1982 Dec;16(6):1061-5. doi: 10.1128/jcm.16.6.1061-1065.1982.
7
Genetic structure of populations of Legionella pneumophila.嗜肺军团菌群体的遗传结构。
J Bacteriol. 1985 Sep;163(3):1021-37. doi: 10.1128/jb.163.3.1021-1037.1985.
8
Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.利用单克隆抗体开发嗜肺军团菌1血清型的标准化亚群分类方案。
J Clin Microbiol. 1986 Apr;23(4):768-71. doi: 10.1128/jcm.23.4.768-771.1986.
9
Legionella species as hospital-acquired respiratory pathogens.军团菌属作为医院获得性呼吸道病原体。
Semin Respir Infect. 1987 Mar;2(1):34-47.
10
Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov.从一名致命肺炎患者身上分离出的嗜肺军团菌血清型兰辛3，以及嗜肺军团菌嗜肺亚种新亚种、嗜肺军团菌弗雷泽亚种新亚种和嗜肺军团菌帕斯库莱亚种新亚种的描述
J Clin Microbiol. 1988 Sep;26(9):1695-703. doi: 10.1128/jcm.26.9.1695-1703.1988.
Zotero 插件