Kim Hyun-Sook, Srinivasan Sathiyaraj, Lee Sang-Seob
Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea.
Curr Microbiol. 2015 Jun;70(6):865-70. doi: 10.1007/s00284-015-0795-9. Epub 2015 Mar 21.
A white-colored bacterial strain designated J20-6(T) was isolated from peat soil collected in Russia. Based on the 16S rRNA gene sequence similarities, the strain J20-6(T) belonged to the genus Paenibacillus, and the closest relatives were Paenibacillus frigoriresistens YIM 016(T) (98.2 %), Paenibacillus alginolyticus DSM 5050T(T) (97.9 %), Paenibacillus chondroitinus DSM 5051(T) (97.4 %), Paenibacillus pocheonensis Gsoil 1138(T) (96.9 %), and Paenibacillus pectinilyticus RCB-08(T) (96.6 %). Cells are gram-positive, motile, facultative aerobic, endospore forming, and rod shaped. The cell wall contains MK-7 as the predominant menaquinone and meso-diaminopimelic acid as the diagnostic diamino acid. The major fatty acid is anteiso-C15:0, and the major polar lipids are diphosphatidylglycerol and phosphatidyl-ethanolamine. The DNA G+C content of the strain J20-6(T) was 49.9 mol %. The phenotypic, chemotaxonomic, and phylogenetic data clearly suggest that the strain J20-6(T) belongs to the novel member of the genus Paenibacillus, for which the name Paenibacillus alba sp. nov., is proposed. The type strain is J20-6(T) (=KEMC 7302-005(T) = JCM 18165(T)).
从俄罗斯采集的泥炭土中分离出一株白色细菌菌株，命名为J20-6(T)。基于16S rRNA基因序列相似性，菌株J20-6(T)属于芽孢杆菌属，与其亲缘关系最近的是耐冷芽孢杆菌YIM 016(T)（98.2%）、解藻酸芽孢杆菌DSM 5050T(T)（97.9%）、软骨素芽孢杆菌DSM 5051(T)（97.4%）、抱川芽孢杆菌Gsoil 1138(T)（96.9%）和果胶分解芽孢杆菌RCB-08(T)（96.6%）。细胞革兰氏阳性，具运动性，兼性需氧，形成芽孢，呈杆状。细胞壁以MK-7作为主要甲基萘醌，以中-二氨基庚二酸作为特征性二氨基酸。主要脂肪酸为anteiso-C15:0，主要极性脂为双磷脂酰甘油和磷脂酰乙醇胺。菌株J20-6(T)的DNA G+C含量为49.9 mol%。表型、化学分类和系统发育数据清楚地表明，菌株J20-6(T)属于芽孢杆菌属的一个新成员，为此提出新名称白色芽孢杆菌（Paenibacillus alba sp. nov.）。模式菌株为J20-6(T)（=KEMC 7302-005(T) = JCM 18165(T)）。
