Kim Dal-Soo, Bae Cheol-Yong, Jeon Jae-Jin, Chun Sam-Jae, Oh Hyun Woo, Hong Soon Gyu, Baek Keun-Sik, Moon Eun Young, Bae Kyung Sook
LG Life Sciences Ltd/R&D Park, #104-1 Moonji-dong, Yuseong-gu, Daejeon 305-380, Republic of Korea.
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, #52, Oun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2031-2035. doi: 10.1099/ijs.0.02414-0.
Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol%, and the major fatty acid was anteiso-C(15 : 0) (54.1 %). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 % with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 %, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)).
从紫苏根部分离出两株具有广泛抗菌活性的新型产芽孢细菌。分离菌株SD17(T)和SD18为兼性厌氧菌，革兰氏反应可变。在20至45摄氏度之间观察到生长。SD17(T)的DNA G+C含量为51.7 mol%，主要脂肪酸为anteiso-C(15 : 0)(54.1%)。SD17(T)的16S rRNA基因序列与其他类芽孢杆菌属物种的相似性范围为98.6%至91.3%。系统发育树显示，分离株SD17(T)与韩国类芽孢杆菌KCTC 2393(T)和埃希类芽孢杆菌IFO 15659(T)形成了一个显著的单系分支。菌株SD17(T)与韩国类芽孢杆菌KCTC 2393(T)和埃希类芽孢杆菌IFO 15659(T)的DNA-DNA相关性值分别为17.4%和19.8%。因此，这些分离株在类芽孢杆菌属内应具有种的地位，为此提出新种名为埃尔吉类芽孢杆菌（Paenibacillus elgii sp. nov.）。模式菌株为SD17(T)(=KCTC 10016BP(T)=NBRC 100335(T))。
