Fanti Franco, Tortoli Enrico, Hall Leslie, Roberts Glenn D, Kroppenstedt Reiner M, Dodi Icilio, Conti Stefania, Polonelli Luciano, Chezzi Carlo
Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma, 43100 Parma, Italy.
Laboratorio di Microbiologia e Virologia, Ospedale di Careggi, 50139 Florence, Italy.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1123-1127. doi: 10.1099/ijs.0.02760-0.
The isolation and identification of a novel, slow-growing, scotochromogenic, mycobacterial species is reported. A strain, designated MUP 1182T, was isolated from a cervical lymph node of a 3-year-old child. MUP 1182T is alcohol- and acid-fast, with a lipid pattern that is consistent with those of species that belong to the genus Mycobacterium. It grows slowly at 25-37 degrees C, but does not grow at 42 degrees C. The isolate was revealed to be biochemically distinct from previously described mycobacterial species: it has urease and Tween hydrolysis activities and lacks nitrate reductase, 3-day arylsulfatase and beta-glucosidase activities. Comparative 16S rDNA sequencing showed that isolate MUP 1182T represents a novel, slow-growing species that is related closely to Mycobacterium lentiflavum and Mycobacterium simiae. On the basis of these findings, the name Mycobacterium parmense sp. nov. is proposed, with MUP 1182T (=CIP 107385T=DSM 44553T) as the type strain.
报道了一种新型、生长缓慢、产暗色素、分枝杆菌属细菌的分离与鉴定。从一名3岁儿童的颈部淋巴结中分离出一株命名为MUP 1182T的菌株。MUP 1182T抗酸和抗酒精，其脂质模式与分枝杆菌属的细菌一致。它在25-37℃下生长缓慢，但在42℃下不生长。该分离株在生化特性上与先前描述的分枝杆菌属细菌不同：它具有脲酶和吐温水解活性，但缺乏硝酸还原酶、3日芳基硫酸酯酶和β-葡萄糖苷酶活性。16S rDNA序列比较显示，分离株MUP 1182T代表一种新型、生长缓慢的细菌，与淡黄分枝杆菌和猿分枝杆菌密切相关。基于这些发现，提出了新种帕尔马分枝杆菌的名称，以MUP 1182T（=CIP 107385T=DSM 44553T）作为模式菌株。
