Yumoto Isao, Hirota Kikue, Kawahara Tomoo, Nodasaka Yoshinobu, Okuyama Hidetoshi, Matsuyama Hidetoshi, Yokota Yuji, Nakajima Kenji, Hoshino Tamotsu
Institute of Biological Resources and Function, Hokkaido Centre, National Institute of Advanced Industrial Science and Technology, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Minaminosawa, Minami-ku, Sapporo 005-8601, Japan.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1239-1242. doi: 10.1099/ijs.0.02889-0.
A novel moderately thermophilic bacterium, strain TH13T, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30-64 degrees C (optimum 54 degrees C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for H2S production and growth in more than 3% NaCl (w/v). The isolate grew at pH 7-8, but not at pH values higher than 9. The DNA G+C content was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13T was a member of the genus Anoxybacillus. DNA-DNA hybridization revealed a low relatedness (less than 30.2%) between the isolate and its close phylogenetic neighbours Anoxybacillus pushchinoensis and Anoxybacillus flavithermus. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name Anoxybacillus voinovskiensis sp. nov. is proposed. The type strain of this species is TH13T (=NCIMB 13956T=JCM 12111T).
从堪察加半岛的一个温泉中分离出了一种新型嗜热细菌，菌株TH13T。发现它是革兰氏阳性兼性需氧菌；直的、无运动性的杆菌在30-64℃（最适温度54℃）下生长。该分离株过氧化氢酶和氧化酶试验呈阳性，能将硝酸盐还原为亚硝酸盐，但不产生硫化氢，在超过3%（w/v）的NaCl中不生长。该分离株在pH 7-8时生长，但在pH值高于9时不生长。DNA G+C含量为43.9 mol%。基于16S rRNA基因测序的系统发育分析表明，菌株TH13T是嗜热栖热放线菌属的一个成员。DNA-DNA杂交显示该分离株与其近缘系统发育邻种嗜热栖热放线菌和嗜热栖热放线菌之间的相关性较低（低于30.2%）。根据表型特征、系统发育数据和DNA-DNA杂交数据，得出该分离株应被分类为一个新物种的结论，为此提出了嗜热栖热放线菌新种的名称。该物种的模式菌株是TH13T（=NCIMB 13956T=JCM 12111T）。
