Yoon Jung-Hoon, Kim In-Gi, Schumann Peter, Oh Tae-Kwang, Park Yong-Ha
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1317-1321. doi: 10.1099/ijs.0.02779-0.
A Gram-positive, motile, round to ellipsoidal, endospore-forming, rod-shaped bacterial strain, SF-57T, was isolated from a marine solar saltern in Korea. This organism grew between 4 and 39 degrees C, with optimum growth at 30 degrees C. Strain SF-57T grew in the presence of 0.5-15.0% NaCl, with optimum growth at 2-3% NaCl. The peptidoglycan type of strain SF-57T was A1alpha linked directly through l-Lys. In strain SF-57TT, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and anteiso-C(15 : 0) was the major fatty acid. The DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SF-57T formed a coherent cluster with Marinibacillus marinus, with a bootstrap resampling value of 100%. The level of 16S rRNA gene sequence similarity between strain SF-57T and M. marinus DSM 1297T was 98.9%. The mean DNA-DNA relatedness level between strain SF-57T and the type strain of M. marinus was 20.6%. Based on phenotypic properties, phylogenetic analyses and genomic data, strain SF-57T merits placement in the genus Marinibacillus as a representative of a novel species, for which the name Marinibacillus campisalis sp. nov. is proposed. The type strain is SF-57T (=KCCM 41644T=JCM 11810T).
从韩国的一个海洋太阳能盐场中分离出了一株革兰氏阳性、具运动性、圆形至椭圆形、产芽孢的杆状细菌菌株SF-57T。该菌株在4至39℃之间生长，最适生长温度为30℃。菌株SF-57T在0.5 - 15.0% NaCl存在的情况下生长，最适生长NaCl浓度为2 - 3%。菌株SF-57T的肽聚糖类型为通过l-Lys直接连接的A1α型。在菌株SF-57T中，甲基萘醌-7（MK-7）是主要的类异戊二烯醌，anteiso-C(15 : 0)是主要脂肪酸。DNA G+C含量为41.8 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株SF-57T与海栖海芽孢杆菌形成了一个紧密的聚类群，自展重抽样值为100%。菌株SF-57T与海栖海芽孢杆菌DSM 1297T的16S rRNA基因序列相似性水平为98.9%。菌株SF-57T与海栖海芽孢杆菌模式菌株的平均DNA-DNA相关性水平为20.6%。基于表型特征、系统发育分析和基因组数据，菌株SF-57T作为一个新物种的代表，有资格归入海芽孢杆菌属，为此提出了新物种名称海栖海芽孢杆菌（Marinibacillus campisalis sp. nov.）。模式菌株为SF-57T（=KCCM 41644T=JCM 11810T）。
