Ferris J P, Ertem G
Department of Chemistry, Rensselaer Polytechnic Institute, Troy, NY 12180.
Science. 1992 Sep 4;257(5075):1387-9. doi: 10.1126/science.1529338.
The regiospecific formation of oligomers from unblocked monomers in aqueous solution is one of the central tenets in research on the origins of life on earth. Direct experimental support for this hypothesis has been obtained in studies of the condensation of the 5'-phosphorimidazolide of adenosine (ImpA) with itself and with P1,P2-diadenosine-5',5'-pyrophosphate (AppA) in water in the presence of a montmorillonite clay. Oligomers of up to ten nucleotides in length are formed. Analysis of the trimers, tetramers, and pentamers formed from a 9:1 ImpA:AppA mixture has shown that 85% of the bonds formed are 3',5'-linked and that any 2',5'-linkages present are at the phosphodiester bond next to the 3'-terminus of the oligomers.
在水溶液中由未封闭的单体区域特异性形成低聚物是地球生命起源研究的核心原则之一。在蒙脱石粘土存在的情况下，对腺苷的5'-磷酰咪唑（ImpA）自身以及与P1，P2-二腺苷-5'，5'-焦磷酸（AppA）在水中缩合的研究中，已经获得了对这一假设的直接实验支持。形成了长度达十个核苷酸的低聚物。对由9:1的ImpA:AppA混合物形成的三聚体、四聚体和五聚体的分析表明，形成的键中有85%是3'，5'-连接的，并且任何存在的2'，5'-连接都位于低聚物3'-末端旁边的磷酸二酯键处。
