没有证据表明粗皮渍螈体内的河豚毒素源于内共生细菌。

No evidence for an endosymbiotic bacterial origin of tetrodotoxin in the newt Taricha granulosa.

作者信息

Lehman Elizabeth M, Brodie Edmund D, Brodie Edmund D

机构信息

Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.

出版信息

Toxicon. 2004 Sep 1;44(3):243-9. doi: 10.1016/j.toxicon.2004.05.019.

DOI:10.1016/j.toxicon.2004.05.019

PMID:15302530

Abstract

Tetrodotoxin (TTX) is a potent neurotoxin which is known to occur in numerous taxa, including newts. The origin of TTX is unknown, but production by symbiotic bacteria is suspected for some groups. Using PCR primers that specifically amplify 16S rRNA genes of bacteria, we examined tissues from rough-skin newts, Taricha granulosa, for the presence of bacteria which may produce TTX. No amplification of bacterial DNA was seen in samples taken from skin, liver, gonads or oviposited eggs-tissues known to contain TTX. Amplification of bacterial DNA was seen only in samples taken from newt intestines, a tissue with low concentrations of TTX. These results indicate that symbiotic bacteria are unlikely to be the source of TTX in newts.

摘要

河豚毒素（TTX）是一种强效神经毒素，已知存在于包括蝾螈在内的众多生物分类群中。TTX的起源尚不清楚，但怀疑某些群体中的TTX是由共生细菌产生的。我们使用特异性扩增细菌16S rRNA基因的PCR引物，检测了粗皮蝾螈（Taricha granulosa）的组织，以寻找可能产生TTX的细菌。在取自皮肤、肝脏、性腺或产卵（已知含有TTX的组织）的样本中未检测到细菌DNA的扩增。仅在取自蝾螈肠道（TTX浓度较低的组织）的样本中检测到细菌DNA的扩增。这些结果表明，共生细菌不太可能是蝾螈体内TTX的来源。