Whitehouse D B, Putt W, Lovegrove J U, Morrison K, Hollyoake M, Fox M F, Hopkinson D A, Edwards Y H
Medical Research Council Human Biochemical Genetics Unit, Galton Laboratory, University College London, United Kingdom.
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):411-5. doi: 10.1073/pnas.89.1.411.
A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows that the homologies between human and rabbit PGM1 mRNAs are 92% and 97% for the coding nucleotide sequence and the amino acid sequence, respectively. The derived rabbit amino acid sequence is in complete agreement with the published protein sequence for rabbit muscle PGM. A physical localization of the human PGM1 gene to chromosome 1p31 has been determined by in situ hybridization. Analysis of DNA from a wide variety of vertebrates indicates a high level of PGM1 sequence conservation during evolution.
已分离并鉴定出一个编码高度多态性人类酶磷酸葡萄糖变位酶1（PGM1；EC 5.4.2.2）mRNA的cDNA克隆。这是通过首先使用抗兔PGM抗体从表达文库中分离出兔cDNA间接实现的。核苷酸序列比较表明，人类和兔PGM1 mRNA的编码核苷酸序列和氨基酸序列的同源性分别为92%和97%。推导的兔氨基酸序列与已发表的兔肌肉PGM蛋白质序列完全一致。通过原位杂交确定了人类PGM1基因在染色体1p31上的物理定位。对多种脊椎动物DNA的分析表明，在进化过程中PGM1序列具有高度保守性。
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