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严重急性呼吸综合征相关冠状病毒3a蛋白在感染的非洲绿猴肾细胞E6和严重急性呼吸综合征患者中的特性分析

Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.

作者信息

Zeng Rong, Yang Rui-Fu, Shi Mu-De, Jiang Man-Rong, Xie You-Hua, Ruan Hong-Qiang, Jiang Xiao-Sheng, Shi Lv, Zhou Hu, Zhang Lei, Wu Xiao-Dong, Lin Ying, Ji Yong-Yong, Xiong Lei, Jin Yan, Dai Er-Hei, Wang Xiao-Yi, Si Bin-Ying, Wang Jin, Wang Hong-Xia, Wang Cui-E, Gan Yong-Hua, Li Yu-Chuan, Cao Ju-Tian, Zuo Jiang-Ping, Shan Shi-Fang, Xie En, Chen Song-Hua, Jiang Zhi-Qin, Zhang Xi, Wang Yuan, Pei Gang, Sun Bing, Wu Jia-Rui

机构信息

Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.

出版信息

J Mol Biol. 2004 Jul 30;341(1):271-9. doi: 10.1016/j.jmb.2004.06.016.

DOI:10.1016/j.jmb.2004.06.016
PMID:15312778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7127270/
Abstract

Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment.

摘要

蛋白质组学被用于鉴定严重急性呼吸综合征相关冠状病毒（SARS-CoV）中开放阅读框3a（ORF 3a）编码的一种蛋白质。免疫印迹显示该蛋白质与刺突蛋白之间可能形成链间二硫键。酶联免疫吸附测定表明，SARS患者的血清与源自3a蛋白的合成肽有显著阳性反应。这些结果与源自刺突蛋白的肽的结果一致。SARS-CoV分离株的3a蛋白和刺突蛋白之间存在共同突变的趋势，表明3a蛋白的功能与刺突蛋白相关。综上所述，3a蛋白在SARS-CoV的功能中可能与刺突蛋白紧密相关。3a蛋白可能成为SARS治疗的新临床标志物或药物靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/362afc2f4af7/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/648aa1079387/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/0b5dc2ce7471/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/b880b4849da6/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/362afc2f4af7/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/648aa1079387/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/0b5dc2ce7471/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/b880b4849da6/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a426/7127270/362afc2f4af7/gr4_lrg.jpg

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