Schreiter Jeannine, Meyer Sophia, Schmidt Christian, Schulz Ronny M, Langer Stefan
Department of Plastic, Aesthetic and Special Hand Surgery, Clinic and Polyclinic for Orthopaedics, Traumatology and Plastic Surgery, University Hospital Leipzig, Germany.
Centre for Biotechnology and Biomedicine, Leipzig, Germany.
GMS Interdiscip Plast Reconstr Surg DGPW. 2017 Jul 10;6:Doc10. doi: 10.3205/iprs000112. eCollection 2017.
: The use of dorsal skinfold chamber models has substantially improved the understanding of micro-vascularisation in pathophysiology over the last eight decades. It allows pathophysiological studies of vascularisation over a continuous period of time. The dorsal skinfold chamber is an attractive technique for monitoring the vascularisation of autologous or allogenic transplants, wound healing, tumorigenesis and compatibility of biomaterial implants. To further reduce the animals' discomfort while carrying the dorsal skinfold chamber, we developed a smaller chamber (the Leipzig Dorsal Skinfold Chamber) and summarized the commercial available chamber models. In addition we compared our model to the common chamber. The Leipzig Dorsal Skinfold Chamber was applied to 66 C57Bl/6 female mice with a mean weight of 22 g. Angiogenesis within the dorsal skinfold chamber was evaluated after injection of fluorescein isothiocyanate dextran with an Axio Scope microscope. The mean vessel density within the dorsal skinfold chamber was assessed over a period of 21 days at five different time points. The gained data were compared to previous results using a bigger and heavier dorsal skinfold model in mice. A PubMed and a patent search were performed and all papers related to "dorsal skinfold chamber" from 1 of January 2006 to 31 of December 2015 were evaluated regarding the dorsal skinfold chamber models and their technical improvements. The main models are described and compared to our titanium Leipzig Dorsal Skinfold Chamber model. The Leipzig Dorsal Skinfold Chamber fulfils all requirements of continuous models known from previous chamber models while reducing irritation to the mice. Five different chamber models have been identified showing substantial regional diversity. The newly elaborated titanium dorsal skinfold chamber may replace the pre-existing titanium chamber model used in Germany so far, as it is smaller and lighter than the former ones. However, the new chamber does not reach the advantages of already existing chamber models used in Asia and the US, which are smaller and lighter. Elaborating a smaller and lighter dorsal skinfold chamber allows research studies on smaller animals and reduces the animals' discomfort while carrying the chamber. Greater research exchange should be done to spread the use of smaller and lighter chamber models.
在过去的八十年里，背部皮褶腔模型的使用极大地增进了我们对病理生理学中微血管形成的理解。它能够在一段连续的时间内进行血管形成的病理生理学研究。背部皮褶腔是一种用于监测自体或异体移植的血管形成、伤口愈合、肿瘤发生以及生物材料植入物相容性的有吸引力的技术。为了在动物携带背部皮褶腔时进一步减轻它们的不适，我们开发了一种更小的腔室（莱比锡背部皮褶腔）并总结了市面上可用的腔室模型。此外，我们将我们的模型与常见的腔室进行了比较。莱比锡背部皮褶腔应用于66只平均体重为22克的C57Bl/6雌性小鼠。在注射异硫氰酸荧光素葡聚糖后，使用Axio Scope显微镜评估背部皮褶腔内的血管生成。在21天的时间里，在五个不同的时间点评估背部皮褶腔内平均血管密度。将获得的数据与之前使用更大、更重的小鼠背部皮褶模型得到的结果进行比较。进行了PubMed和专利检索，并对2006年1月1日至2015年12月31日期间所有与“背部皮褶腔”相关的论文中关于背部皮褶腔模型及其技术改进的内容进行了评估。描述了主要模型并将其与我们的钛制莱比锡背部皮褶腔模型进行比较。莱比锡背部皮褶腔满足了先前腔室模型中连续模型的所有要求，同时减少了对小鼠的刺激。已确定有五种不同的腔室模型，显示出显著的区域差异。新精心制作的钛制背部皮褶腔可能会取代迄今为止德国使用的先前钛腔室模型，因为它比前者更小、更轻。然而，新腔室并未达到亚洲和美国现有腔室模型的优点，后者更小、更轻。精心制作更小、更轻的背部皮褶腔可以对更小的动物进行研究，并减少动物携带腔室时的不适。应该进行更多的研究交流，以推广使用更小、更轻的腔室模型。
