D'Annibale Alessandro, Ricci Marika, Quaratino Daniele, Federici Federico, Fenice Massimiliano
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, Italy.
Res Microbiol. 2004 Sep;155(7):596-603. doi: 10.1016/j.resmic.2004.04.009.
This study was aimed at assessing the potential of the white-rot fungus Panus tigrinus CBS 577.79 in removing organic load, color and toxic phenols from agro-industrial effluent olive-mill wastewater (OMW). The influence of wastewater composition on P. tigrinus degradative capability was investigated in shaken cultures using two different OMWs. The initial soluble COD of 85,000 mg l(-1) led to a delay in removal of color, organic load and phenol by the fungus. This was associated with delayed onset of laccase and Mn-dependent peroxidase. On the other hand, P. tigrinus, when grown on OMW with an initial soluble COD content of 43,000 mg l(-1), promptly and efficiently removed the aforementioned components. Chromatographic analyses showed that 4-hydroxy-substituted simple phenols were predominantly removed. The polymeric aromatic fraction underwent simultaneous polymerization and depolymerization. This study is a contribution to the understanding of the degradative specificity of P. tigrinus on OMW aromatic components and provides good indications for possible future applications of the fungus.
本研究旨在评估白腐真菌虎皮香菇CBS 577.79从农业工业废水橄榄果渣废水（OMW）中去除有机负荷、颜色和有毒酚类的潜力。在摇瓶培养中，使用两种不同的OMW研究了废水组成对虎皮香菇降解能力的影响。初始可溶性化学需氧量（COD）为85,000 mg l(-1)导致真菌去除颜色、有机负荷和酚类的过程延迟。这与漆酶和锰依赖性过氧化物酶的延迟产生有关。另一方面，当虎皮香菇在初始可溶性COD含量为43,000 mg l(-1)的OMW上生长时，能迅速有效地去除上述成分。色谱分析表明，主要去除的是4-羟基取代的简单酚类。聚合物芳香族部分同时发生聚合和解聚。本研究有助于理解虎皮香菇对OMW芳香族成分的降解特异性，并为该真菌未来可能的应用提供了良好的指示。
