Quaratino Daniele, D'Annibale Alessandro, Federici Federico, Cereti Carlo Fausto, Rossini Francesco, Fenice Massimiliano
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via San Camillo de Lellis snc I-01100 Viterbo, Italy.
Chemosphere. 2007 Jan;66(9):1627-33. doi: 10.1016/j.chemosphere.2006.07.092. Epub 2006 Sep 27.
The phytotoxicity of olive-mill wastewater (OMW) has been suggested to be mainly due to its phenolic components. This study investigated the impact of three different low-cost dephenolization treatments on the wastewater phytotoxicity. To this aim, germinability of maize (Zea mays L.) seeds sown on a sandy-loamy soil which had been spread with different volumes (from 40 to 160m(3)ha(-1)) of either biologically-treated OMW or relative incubation control was determined. Biological treatments included either Panus tigrinus liquid cultures or incubation with commercial laccase (1UIml(-1)) or an innovative sequential combination of laccase and P. tigrinus cultures. All treatments markedly reduced phytotoxicity and promising results were obtained with commercial laccase. In fact, germinability and mean germination times in soil spread with laccase-treated OMW, did not significantly differ from those observed in soil irrigated with tap water (control) up to OMW volumes of 120m(3)ha(-1). Although the highest phenol reduction (ca. 81%) was obtained by the sequential use of laccase and P. tigrinus, the feasibility of the enzyme treatment is undoubtedly more convincing under the technological point of view.
橄榄油厂废水（OMW）的植物毒性被认为主要归因于其酚类成分。本研究调查了三种不同的低成本脱酚处理对废水植物毒性的影响。为此，测定了播种在砂壤土上的玉米（Zea mays L.）种子的发芽率，该砂壤土已施加了不同体积（从40至160 m³·ha⁻¹）的经过生物处理的OMW或相应的培养对照。生物处理包括豹斑多孔菌液体培养物，或与商业漆酶（1 U·ml⁻¹）一起培养，或漆酶与豹斑多孔菌培养物的一种创新的顺序组合。所有处理均显著降低了植物毒性，并且使用商业漆酶获得了有前景的结果。事实上，在施加了用漆酶处理过的OMW的土壤中，直至OMW体积达到120 m³·ha⁻¹时，发芽率和平均发芽时间与用自来水灌溉的土壤（对照）中观察到的情况没有显著差异。尽管通过顺序使用漆酶和豹斑多孔菌获得了最高的酚类去除率（约81%），但从技术角度来看，酶处理的可行性无疑更具说服力。
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