Aggelis G, Iconomou D, Christou M, Bokas D, Kotzailias S, Christou G, Tsagou V, Papanikolaou S
Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece.
Water Res. 2003 Sep;37(16):3897-904. doi: 10.1016/S0043-1354(03)00313-0.
Pleurotus ostreatus grown in bioreactor batch cultures in a model phenolic wastewater (diluted and sterilized olive oil mill wastewater-OMW), caused significant phenolic removal. Laccase, the sole ligninolytic enzyme detected in the growth environment, was produced during primary metabolic growth. The bioprocess was simulated with the aid of a mathematical model and the parameters of growth were determined. When the fungal biomass was increased in the reactor (during repeated batch experiments) the rate of reducing sugars consumption progressively increased, but a phenolic fraction seemed of being strongly resistant to oxidation. The toxicity of OMW against the seeds of Lepidium sativum and the marine Branchiopoda Artemia sp. was significantly decreased after biotreatment. On the contrary, the toxicity against the freshwater Branchiopoda Daphnia magna was not affected by the treatment, whereas on the soil and freshwater sediments Ostracoda Heterocypris incongruens was slightly decreased. Both treated and untreated OMWs, used as water for irrigation of lettuce and tomato plants, did not significantly affect the uptake of several nutrients by the cultivated plants, but resulted in a decrease in the plant yields, which was minimized when high OMW dilutions were used. As a conclusion, P. ostreatus is able to reduce phenolic content and toxicity of sterilized OMW, in bioreactor cultures. However, high OMW dilutions should be used, and/or additional treatment should be applied before use of the OMW in the environment, e.g. as water for irrigation. Further research should be done in order to transfer this technology under industrial conditions (e.g. by using unsterilized OMW).
在模拟酚类废水（稀释并灭菌的橄榄油压榨废水-OMW）的生物反应器分批培养中生长的平菇，能显著去除酚类物质。漆酶是在生长环境中检测到的唯一木质素分解酶，在初级代谢生长过程中产生。借助数学模型对生物过程进行了模拟，并确定了生长参数。当反应器中真菌生物量增加时（在重复分批实验期间），还原糖消耗速率逐渐增加，但有一部分酚类物质似乎对氧化具有很强的抗性。经生物处理后，OMW对独行菜种子和海洋鳃足亚纲卤虫的毒性显著降低。相反，对淡水鳃足亚纲大型溞的毒性不受处理影响，而对土壤和淡水沉积物中的异足介形虫的毒性略有降低。无论是经处理还是未经处理的OMW，用作生菜和番茄植株的灌溉水时，对栽培植物吸收几种养分的影响均不显著，但会导致作物产量下降，当使用高稀释度的OMW时，这种下降会降至最低。总之，平菇能够在生物反应器培养中降低灭菌OMW的酚类含量和毒性。然而，应使用高稀释度的OMW，和/或在将OMW用于环境（如作为灌溉水）之前进行额外处理。为了在工业条件下（如使用未灭菌的OMW）应用这项技术，还应开展进一步研究。
