Hanif A, Bakopoulos V, Dimitriadis G J
Division of Genetics, Department of Biology, University of Patras, Patras 26500, Greece.
Fish Shellfish Immunol. 2004 Nov;17(5):411-35. doi: 10.1016/j.fsi.2004.04.013.
Immunisation of sea bream (Sparus aurata L.) broodstock with a novel vaccine mixture of Photobacterium damsela subsp. piscicida SK7 (Phdp) was performed during the period of egg development and the changes in specific and non-specific humoral immune parameters were measured. Total immunoglobulin level, specific antibody titre, anti-protease activity and lysozyme activity were significantly higher in immunised parents compared to the control. After spawning significantly higher anti-protease activity, lysozyme activity and total immunoglobulin level were detected in the eggs from immunised parents. Specific antibody titres against Phdp were only detected in the eggs from the immunised parents. The larvae from immunised parents also expressed significantly higher levels of specific and non-specific humoral immune parameters compared to the controls. A small amount of total immunoglobulin was detected in larvae decreasing gradually until day 8 post-hatching and then an increase was measured in larvae from immunised parents, whereas no immunoglobulin was detected at days 4, 6 and 8 in larvae from non-immunised parents. The specific antibody titre against Phdp was detected only in larvae from immunised broodstock until day 14 post-hatching. The higher humoral immune parameters in eggs and larvae from immunised parents in comparison to eggs and larvae from non-immunised parents, suggest transfer of maternal specific and non-specific immune factors.
在卵形鲳鲹（Sparus aurata L.）亲鱼的卵子发育期间，用新型疫苗混合物美人鱼发光杆菌杀鱼亚种SK7（Phdp）进行免疫接种，并测量特异性和非特异性体液免疫参数的变化。与对照组相比，免疫亲鱼的总免疫球蛋白水平、特异性抗体滴度、抗蛋白酶活性和溶菌酶活性显著更高。产卵后，在免疫亲鱼的卵子中检测到显著更高的抗蛋白酶活性、溶菌酶活性和总免疫球蛋白水平。仅在免疫亲鱼的卵子中检测到针对Phdp的特异性抗体滴度。与对照组相比，免疫亲鱼的幼体也表达出显著更高水平的特异性和非特异性体液免疫参数。在幼体中检测到少量总免疫球蛋白，其含量在孵化后第8天之前逐渐下降，然后在免疫亲鱼的幼体中检测到含量增加，而在未免疫亲鱼的幼体中，在第4、6和8天未检测到免疫球蛋白。直到孵化后第14天，仅在免疫亲鱼的幼体中检测到针对Phdp的特异性抗体滴度。与未免疫亲鱼的卵子和幼体相比，免疫亲鱼的卵子和幼体中更高的体液免疫参数表明母体特异性和非特异性免疫因子发生了转移。
