Sato H, Hirose K, Saito H
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Vet Microbiol. 1995 Feb;43(2-3):173-82. doi: 10.1016/0378-1135(95)92533-h.
The protective activity in mice and antigenic composition of the culture filtrate of 11 strains of Erysipelothrix rhusiopathiae were compared. Protective activity was found in the first (P-1) fraction obtained by Sephadex G-200 gel filtration of the culture filtrate of each strain. Comparing the 50% protective dose (PD50) of the P-1 fraction of the 11 strains by active immunization, highly protective activity was shown by 5 strains, such as Agata, Fujisawa, Shizuoka-63, Koganei 65-0.15 and SE-9. For the 50% effective dose (ED50) determined by passive protective studies, the four strains, Agata, Fujisawa, Koganei 65-0.15 and SE-9 were shown to be highly protective. However, strains 2179 and 2553 showed low activities in both PD50 and ED50. The highly and weakly protective strains were compared by western blot analysis for the protein components. In most strains tested, there were two protein bands of molecular weight of 64 kDa and 43 kDa. Therefore, these two structural proteins were common to the strains and were associated with stimulation of a protective effect in mice.
比较了11株猪丹毒杆菌培养滤液在小鼠体内的保护活性及其抗原组成。在通过Sephadex G - 200凝胶过滤各菌株培养滤液得到的第一部分（P - 1）中发现了保护活性。通过主动免疫比较11株菌株P - 1部分的50%保护剂量（PD50），Agata、藤泽、静冈 - 63、小金井65 - 0.15和SE - 9等5株菌株表现出高保护活性。对于通过被动保护研究确定的50%有效剂量（ED50），Agata、藤泽、小金井65 - 0.15和SE - 9这4株菌株显示出高保护作用。然而，2179和2553菌株在PD50和ED50方面均表现出低活性。通过蛋白质印迹分析比较高保护和低保护菌株的蛋白质成分。在大多数测试菌株中，有两条分子量分别为64 kDa和43 kDa的蛋白带。因此，这两种结构蛋白是菌株共有的，并且与小鼠体内保护作用的刺激相关。
