Rochkind S, Kogan G, Luger E G, Salame K, Karp E, Graif M, Weiss J
Department of Neurosurgery, Division of Peripheral Nerve Reconstruction, Tel Aviv Sourasky Medical Center, Tel Aviv University, Israel.
Photomed Laser Surg. 2004 Jun;22(3):249-53. doi: 10.1089/1549541041438579.
We investigated the therapeutic efficiency of laser irradiation and Bio-Oss, both and separately, on the post-traumatic regeneration of bone tissue in rats using infrared spectroscopy as an informative and accurate measuring method.
The therapeutic effect of low-power laser irradiation on bone tissue regeneration processes in animal models has been studied using morphogenic, biochemical, roentgenographic and electron microscopic measurements. Natural bone minerals, such as Bio-Oss collagen, were recommended for the reconstruction of bone defects in the alveolar process.
29 male Wistar rats, divided into four random groups in a blinded manner were operated on the right alveolar process. A bone defect was made by penetrating the right alveolar process of the mandible bone using a 3-mm drill. The rats were divided into four groups as follows: Group I, left side served as intact bone and right injured side as the control; Group II, right injured side was treated by organic bovine bone (Bio-Oss); Group III, right side bone defect was treated by HeNe laser (632.8 nm, 35 mW) applied transcutaneously for 20 min to the injured area daily for the following 14 consecutive days; and Group IV, Bio-Oss was placed loosely in the right side defect followed by laser treatment. After 2 weeks, the intact bone and bone replicas of the trauma area were removed and analyzed by infra-red spectroscopy technique. The composition and the structure of the bone tissue mineral substances were determined and compared among the four groups. For quantitative analysis of the regenerative bone process, the Mineralization index was used. An increase in this index indicates regenerative bone processes.
The normal state analysis of the IR spectra of the normal alveolar bone tissue within the intervals of 400 to 4000 cm(-1) revealed characteristic absorption bands for the inorganic bone component in spectrum regions 450-1480 cm(-1), and the organic component at 1540-3340 cm(-1). In the case of trauma, the intensity of absorption of the inorganic component was decreased by 54%, and the absorption band became narrow, which can be interpreted as quantitative changes of the bone tissue mineral content. The wavelength characteristics of the inorganic component remained unchanged; that is, the induced trauma under these experimental conditions did not provoke alterations in the structure of the phosphate framework. The organic component showed decreased absorption by 10-15%, compared to the normal bone, and slight displacement of the wavelength, which can be interpreted as changes occurring in the quality of the organic content of the bone tissue. In the Bio-Oss-treated group, the intensity of absorption of the inorganic component increased by 43%, compared to the control injured area; however, there was a decrease of 22.6% in the normal bone. The wavelength characteristics of the inorganic component remained unchanged. The organic component showed similar absorption results in the injured non-treated group and absorption was 10-15% less than in the normal bone. Mineralization Index in the Bio-Oss-treated group was 0.93, compared to 0.63 in the control group and 2.04 in the normal bone. In the laser-treated group, the intensity of absorption of the inorganic component increased by 62, compared to the control injured area, and decreased only 11.4% in the normal bone. The wavelength characteristics of the organic component remained unchanged; that is, the organic component was similar to that of normal bone. Mineralization Index in the laser-treated group increased significantly to 1.86, compared to 0.63 in the control group and 2.04 in the normal bone. In the combined laser and Bio-Oss-treated groups, the intensity of absorption of the inorganic component and organic component was similar to that of normal bone. Mineralization Index in this group increased significantly to 1.98, compared to 0.63 in the control group and 2.04 in the normal bone.
The results suggest that low-power laser irradiatults suggest that low-power laser irradiation alone and in combination with Bio-Oss enhances bone healing and increases bone repair.
我们使用红外光谱作为一种信息丰富且准确的测量方法，研究了激光照射和Bio-Oss单独及联合使用对大鼠创伤后骨组织再生的治疗效果。
已使用形态学、生化、X射线和电子显微镜测量方法研究了低功率激光照射对动物模型中骨组织再生过程的治疗效果。天然骨矿物质，如Bio-Oss胶原蛋白，被推荐用于牙槽突骨缺损的重建。
29只雄性Wistar大鼠，以盲法随机分为四组，对其右侧牙槽突进行手术。使用3毫米钻头穿透下颌骨的右侧牙槽突制造骨缺损。大鼠分为以下四组：第一组，左侧作为完整骨，右侧受伤侧作为对照；第二组，右侧受伤侧用有机牛骨（Bio-Oss）治疗；第三组，右侧骨缺损用氦氖激光（632.8 nm，35 mW）经皮每天照射受伤区域20分钟，持续14天；第四组，在右侧缺损处松散放置Bio-Oss，然后进行激光治疗。2周后，取出完整骨和创伤区域的骨复制品，采用红外光谱技术进行分析。测定并比较四组骨组织矿物质的组成和结构。为了对再生骨过程进行定量分析，使用了矿化指数。该指数的增加表明有再生骨过程。
在400至4000 cm(-1)区间内对正常牙槽骨组织的红外光谱进行正常状态分析，发现在450 - 1480 cm(-1)光谱区域有无机骨成分的特征吸收带，在1540 - 3340 cm(-1)有有机成分的特征吸收带。在创伤情况下，无机成分的吸收强度降低了54%，吸收带变窄，这可解释为骨组织矿物质含量的定量变化。无机成分的波长特征保持不变；也就是说，在这些实验条件下诱导的创伤未引起磷酸盐框架结构的改变。与正常骨相比，有机成分的吸收降低了10 - 15%，且波长有轻微位移，这可解释为骨组织有机成分质量发生的变化。在Bio-Oss治疗组中，与对照受伤区域相比，无机成分的吸收强度增加了43%；然而，与正常骨相比降低了22.6%。无机成分的波长特征保持不变。有机成分在未治疗的受伤组中显示出类似的吸收结果，且比正常骨少10 - 15%。Bio-Oss治疗组的矿化指数为0.93，对照组为0.63，正常骨为2.04。在激光治疗组中，与对照受伤区域相比，无机成分的吸收强度增加了62，与正常骨相比仅降低了11.4%。有机成分的波长特征保持不变；也就是说，有机成分与正常骨相似。激光治疗组的矿化指数显著增加至1.86，对照组为0.63，正常骨为2.04。在激光和Bio-Oss联合治疗组中，无机成分和有机成分的吸收强度与正常骨相似。该组的矿化指数显著增加至1.98，对照组为0.63，正常骨为2.04。
结果表明，低功率激光单独照射以及与Bio-Oss联合使用可促进骨愈合并增加骨修复。
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