Wang Qing-Min, Sun Shu-Han, Hu Zhen-Lin, Yin Ming, Xiao Cun-Jie, Zhang Jian-Cheng
Department of Medical Genetics, The Second Military Medical University, Xiang'Yin Road 800, Shanghai 200433, China.
Vaccine. 2004 Sep 9;22(27-28):3622-7. doi: 10.1016/j.vaccine.2004.03.029.
The study evaluated the immune response elicited by a DNA vaccine encoding ESAT6 protein of Mycobacterium tuberculosis by DNA prime-protein boost protocol. BALB/c mice were respectively vaccinated with plasmid DNA encoding ESAT6 protein, with ESAT6 protein in IFA adjuvant, or a combined DNA prime-protein boost regimen. While DNA immunization induced Th1-polarized immune response, protein-in-adjuvant vaccination elicited a Th2-dominant response. When animals were primed with DNA and boost with protein, both antibodies and Th-cell proliferative response were significantly enhanced. Moreover, production of Th1-type cytokine (IFN-gamma) was increased significantly by DNA priming-protein boosting. This protocol also resulted in an increased relative ratio of IgG2a to IgG1 and the cytotoxicity of T cells. Thus, this study demonstrated that the formation of ESAT6 DNA prime-protein boost inoculation could improved antigen-specific cellular immune responses, which are important for protection against TB infection.
该研究通过DNA初免-蛋白加强方案评估了编码结核分枝杆菌ESAT6蛋白的DNA疫苗引发的免疫反应。将BALB/c小鼠分别用编码ESAT6蛋白的质粒DNA、含ESAT6蛋白的IFA佐剂或联合DNA初免-蛋白加强方案进行免疫接种。虽然DNA免疫诱导了Th1极化的免疫反应，但佐剂蛋白疫苗接种引发了以Th2为主的反应。当用DNA进行初免并用蛋白进行加强时，抗体和Th细胞增殖反应均显著增强。此外，DNA初免-蛋白加强显著增加了Th1型细胞因子（IFN-γ）的产生。该方案还导致IgG2a与IgG1的相对比例增加以及T细胞的细胞毒性增加。因此，本研究表明，ESAT6 DNA初免-蛋白加强接种的形成可改善抗原特异性细胞免疫反应，这对于预防结核感染很重要。
