Kim O T, Kim M Y, Hong M H, Ahn J C, Hwang B
Department of Biology, Chonnam National University, Gwangju, 500-757, South Korea.
Plant Cell Rep. 2004 Nov;23(5):339-44. doi: 10.1007/s00299-004-0826-7. Epub 2004 Aug 14.
The effects of a number of different elicitors on asiaticoside production in whole plant cultures of Centella asiatica were studied, including yeast extract, CdCl(2), CuCl(2) and methyl jasmonate (MJ). Only MJ and yeast extract stimulated asiaticoside production--1.53 and 1.41-fold, respectively. Maximum asiaticoside production was achieved following treatment with 0.1 mM MJ (116.8 mg/l). The highest asiaticoside production (342.72 mg/l) was obtained after 36 days of elicitation in cultures treated with 0.1 mM MJ and 0.025 mg/l 1-phenyl-3-(1,2,3-thidiazol-5-yl)urea (TDZ). Interestingly, MJ not only stimulated the production of asiaticoside but also had an important role in the senescence of C. asiatica. Although asiaticoside content did not change when TDZ was added to medium containing an elicitor, TDZ did increase shoot growth of C. asiatica. We discuss the interactive roles of MJ and TDZ in secondary metabolic production and biomass in whole plants of C. asiatica.
研究了多种不同诱导子对积雪草全株培养物中积雪草苷产量的影响，包括酵母提取物、氯化镉（CdCl₂）、氯化铜（CuCl₂）和茉莉酸甲酯（MJ）。只有MJ和酵母提取物刺激了积雪草苷的产生，分别提高了1.53倍和1.41倍。用0.1 mM MJ处理后实现了积雪草苷的最大产量（116.8 mg/l）。在用0.1 mM MJ和0.025 mg/l 1-苯基-3-（1,2,3-噻二唑-5-基）脲（TDZ）处理的培养物中诱导36天后，获得了最高的积雪草苷产量（342.72 mg/l）。有趣的是，MJ不仅刺激了积雪草苷的产生，而且在积雪草的衰老过程中也起重要作用。虽然当TDZ添加到含有诱导子的培养基中时积雪草苷含量没有变化，但TDZ确实增加了积雪草的茎生长。我们讨论了MJ和TDZ在积雪草全株次生代谢产物产生和生物量方面的相互作用。
