Loc Nguyen Hoang, Nhat Nguyen Thi Duy
Institute of Resources, Environment and Biotechnology, Hue University, Hue 47000, Vietnam.
Asian Pac J Trop Biomed. 2013 Oct;3(10):806-10. doi: 10.1016/S2221-1691(13)60159-8. Epub 2013 Sep 4.
To investigate the effects of some culture conditions on production of asiaticoside from centella (Centella asiatica L. Urban) cells cultured in 5-L bioreactor.
The centell cell suspension culture was conducted in 5-L bioreactor to investigate the growth and asiaticoside accumulation under various conditions. Asiaticoside content was determined by HPLC analysis.
The results showed that the cell growth and asiaticoside accumulation peaked after 24 d of culture at an agitation speed of 150 r/min and aeration rate of 2.5 L/min. The cell biomass reached a maximum value of 302.45 g fresh weight (31.45 g dry weight) and growth index of 3.03 with inoculum size of 100 g. However, asiaticoside content was the highest (60.08 mg/g dry weight) when culture was initiated with an inoculum size of 50 g.
The present study found the suitable conditions for growth of centella cells and their asiaticoside production in bioreactor.
研究某些培养条件对在5-L生物反应器中培养的积雪草（Centella asiatica L. Urban）细胞生产积雪草苷的影响。
在5-L生物反应器中进行积雪草细胞悬浮培养，以研究不同条件下的生长和积雪草苷积累情况。通过高效液相色谱分析测定积雪草苷含量。
结果表明，在搅拌速度为150转/分钟、通气速率为2.5升/分钟的条件下培养24天后，细胞生长和积雪草苷积累达到峰值。接种量为100克时，细胞生物量达到最大值302.45克鲜重（31.45克干重），生长指数为3.03。然而，接种量为50克开始培养时，积雪草苷含量最高（60.08毫克/克干重）。
本研究找到了生物反应器中积雪草细胞生长及其积雪草苷生产的合适条件。
