鉴定控制SXT接合转移的操纵子和启动子。
Identification of operators and promoters that control SXT conjugative transfer.
作者信息
Beaber John W, Waldor Matthew K
机构信息
Department of Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Jaharis 425, Boston, MA 02111, USA.
出版信息
J Bacteriol. 2004 Sep;186(17):5945-9. doi: 10.1128/JB.186.17.5945-5949.2004.
Abstract
Transfer of SXT, a Vibrio cholerae-derived integrating conjugative element that encodes multiple antibiotic resistance genes, is repressed by SetR, a lambda434 cI-related repressor. Here we identify divergent promoters between s086 and setR that drive expression of the regulators of SXT transfer. One transcript encodes the activators of transfer, setC and setD. The second transcript codes for SetR and, like the cI transcript of lambda, is leaderless. SetR binds to four operators located between setR and s086; the locations and relative affinities of these sites suggest a model for regulation of SXT transfer.
摘要
SXT是一种源自霍乱弧菌的整合性接合元件，编码多种抗生素抗性基因，其转移受SetR（一种与λ434 cI相关的阻遏物）抑制。在此，我们鉴定出s086和setR之间的不同启动子，它们驱动SXT转移调节因子的表达。一种转录本编码转移激活因子setC和setD。第二种转录本编码SetR，并且与λ的cI转录本一样，没有前导序列。SetR与位于setR和s086之间的四个操纵子结合；这些位点的位置和相对亲和力提示了一种SXT转移调控模型。
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