López-Vales Rubén, García-Alías Guillermo, Forés Joaquim, Navarro Xavier, Verdú Enrique
Neuroplasticity and Regeneration Group, Institute of Neuroscience and Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Spain.
J Neurotrauma. 2004 Aug;21(8):1031-43. doi: 10.1089/0897715041651105.
Olfactory ensheathing cells (OECs) were transplanted in adult rats after photochemical injury of the spinal cord. Rats received either 180,000 OECs suspended in DMEM or DMEM alone. Locomotor ability scored by the BBB-scale, pain sensibility, and motor and somatosensory evoked potentials were evaluated during the first 14 days post-surgery. At 3, 7, and 14 days, 5 rats per day of both groups were perfused and transverse sections from proximal, lesioned and distal spinal cord blocks were stained for COX-2, VEGF, GFAP and lectin. The BBB-score and the amplitude of motor and somatosensory evoked potentials were significantly higher in OEC- than in DMEM-injected animals throughout follow-up, whereas the withdrawal latency to heat noxious stimulus was lower in OEC- than in DMEM-injected rats. The area of preserved spinal cord and the levels of COX-2 and VEGF staining were significantly higher in OEC- than in DMEM-injected rats. GFAP- but no LEC-positive cells expressed COX-2 staining in OEC-transplanted rats. The density of blood vessels was also significantly increased in OEC- with respect to DMEM-injected rats. Our results show that OECs promote functional and morphological preservation of the spinal cord after photochemical injury, increasing neoangiogenesis and up-regulation of COX-2 and VEGF expression in astrocytes.
在成年大鼠脊髓发生光化学损伤后，移植嗅鞘细胞（OECs）。大鼠分别接受悬浮于DMEM中的180,000个OECs或仅接受DMEM。在术后的前14天，通过BBB评分评估运动能力、疼痛敏感性以及运动和体感诱发电位。在第3、7和14天，每天对两组各5只大鼠进行灌注，并对来自脊髓近端、损伤部位和远端节段的横切片进行COX-2、VEGF、GFAP和凝集素染色。在整个随访过程中，接受OECs注射的动物的BBB评分以及运动和体感诱发电位的幅度显著高于接受DMEM注射的动物，而接受OECs注射的大鼠对热有害刺激的撤腿潜伏期低于接受DMEM注射的大鼠。接受OECs注射的大鼠中脊髓保留区域以及COX-2和VEGF染色水平显著高于接受DMEM注射的大鼠。在接受OECs移植的大鼠中，GFAP阳性细胞而非凝集素阳性细胞表达COX-2染色。与接受DMEM注射的大鼠相比，接受OECs注射的大鼠的血管密度也显著增加。我们的结果表明，OECs可促进光化学损伤后脊髓的功能和形态保存，增加新生血管形成，并上调星形胶质细胞中COX-2和VEGF的表达。
