Begum Ferdousi, Islam Kazi Mohammed Didarul, Paul Rathindra Nath, Mehedi Masfique, Rani Shyamole
Development of Biotechnology & Environmental Conservation Centre, House No 90, Road No 11/A, Dhanmondi R/A, Dhaka-1209, Bangladesh.
Indian J Exp Biol. 2003 Dec;41(12):1479-81.
An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained (12.7 shoots per shoot tip) in MS medium containing 1 mg/L BAP and 1 mg/L NAA. Micropropagated shoots were rooted in 1/2 MS medium supplemented with 1 mg/l IBA. This is the first report of in vitro plant propagation of R. dumetorum. In vitro grown plantlets showed a survival rate of 70% after 2 months of transplantation to natural environment.
已开发出一种有效的体外培养多刺茜树（催吐果）嫩枝增殖方案。多刺茜树的种子在MS培养基中于5周内实现体外萌发。随后以茎尖为外植体，在添加不同浓度和组合的BAP（0.5 - 2.0）和NAA（0.0 - 2.0）的MS培养基中进行增殖培养。在含有1 mg/L BAP和1 mg/L NAA的MS培养基中获得了最大嫩枝增殖量（每个茎尖12.7个嫩枝）。微繁殖的嫩枝在添加1 mg/l IBA的1/2 MS培养基中生根。这是关于多刺茜树体外植株繁殖的首次报道。体外培养的植株在移植到自然环境2个月后的成活率为70%。
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