Yokoyama Eiji, Yamagishi Kenzo, Hara Akira
The Agricultural High-Tech Research Center, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan.
FEMS Microbiol Lett. 2004 Aug 15;237(2):205-12. doi: 10.1016/j.femsle.2004.06.033.
This study developed a PCR-based mating-type assay for Clavicipitaceae. PCR primer sets for the mating-type genes MAT1-1-1 and MAT1-2-1 were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, Cordyceps and Epichloë). Most of the clavicipitaceous fungi possessed either MAT1-1-1 or MAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.
本研究开发了一种基于聚合酶链反应（PCR）的麦角菌科交配型检测方法。分别基于保守的α盒和HMG盒的氨基酸序列设计了交配型基因MAT1-1-1和MAT1-2-1的PCR引物对。基于PCR的交配型检测方法可应用于各种麦角菌属（棒束孢属、麦角菌属、虫草属和香柱菌属）。大多数麦角菌科真菌要么拥有MAT1-1-1，要么拥有MAT1-2-1，被认为是异宗配合的。尽管通过交配型检测获得的PCR产物较短，但交配型基因的系统发育树比18S核糖体DNA（rDNA）的系统发育树具有更好的分辨率，并且与它们的生态营养模式非常吻合。
