Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal.
Fungal Biol. 2010 Feb-Mar;114(2-3):255-70. doi: 10.1016/j.funbio.2010.01.007. Epub 2010 Jan 29.
Sexual reproduction in ascomycete fungi is governed by the mating-type (MAT) locus. The MAT loci of Diaporthe and its Phomopsis anamorphs differ in only one gene: MAT1-1-1 in mating-type MAT1-1 and MAT1-2-1 in mating-type MAT1-2. In order to diagnose mating-types in Diaporthe and Phomopsis and evaluate their usefulness in teleomorph induction in vitro and biological species delimitation, we designed primers that amplify part of the MAT1-1-1 and MAT1-2-1 genes. MAT phylogenies were generated and compared with ITS and EF1-α phylograms. Species recognised in the EF1-α phylogeny corresponded directly with those determined in the MAT phylogenies. ITS was shown to be highly variable resulting in a large number of phylogenetic species that were discordant with MAT and EF1-α species. Mating experiments were conducted to evaluate the existence of reproductive barriers between some isolates, and their anamorphic morphologies were compared. The primers proved to be useful in the mating-type diagnosis of isolates, selection of compatible mating pairs, and in the assessment of biological species boundaries.
子囊菌真菌的有性生殖受交配型 (MAT) 基因座控制。在 Diaporthe 及其 Phomopsis 无性型中，MAT 基因座仅在一个基因上存在差异：交配型 MAT1-1 中的 MAT1-1-1 和交配型 MAT1-2 中的 MAT1-2-1。为了在 Diaporthe 和 Phomopsis 中诊断交配型，并评估其在体外同形诱导和生物种界限划分中的有用性，我们设计了扩增 MAT1-1-1 和 MAT1-2-1 基因部分的引物。生成了 MAT 系统发育树，并与 ITS 和 EF1-α 系统发育树进行了比较。在 EF1-α 系统发育树上识别的物种与在 MAT 系统发育树上确定的物种直接对应。ITS 表现出高度变异性，导致大量与 MAT 和 EF1-α 物种不一致的系统发育种。进行了交配实验来评估一些分离物之间是否存在生殖障碍，并比较了它们的无性形态。这些引物被证明在分离物的交配型诊断、相容交配对的选择以及生物种界限的评估中非常有用。
