Sung Jae-Mo, Park Young-Jin, Lee Je-O, Han Sang-Kuk, Lee Won-Ho, Choi Sung-Keun, Shrestha Bhushan
Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University, Chuncheon 200-701, Korea.
Mycobiology. 2006 Sep;34(3):131-7. doi: 10.4489/MYCO.2006.34.3.131. Epub 2006 Sep 30.
In vitro fruiting bodies were produced from ten different isolates of Cordyceps militaris EFCC C-5736, EFCC C-5941, EFCC C-5976, EFCC C-6040, EFCC C-6849, EFCC C-7268, EFCC C-7342, EFCC C-7992, EFCC C-8027 and EFCC C-8549. Single ascospores were isolated from in vitro grown fruiting bodies and used for fruiting body production in brown rice medium by both intra-strain crossing and out-crossing. Length and dry wt. of stromata grown in vitro were measured. Strains producing highest dry wt. of stromata were selected. Both intra-strain crossings and inter-strain crossings of single ascospore strains were found to produce profuse fruiting bodies of C. militaris.
从10种不同的蛹虫草分离株（EFCC C - 5736、EFCC C - 5941、EFCC C - 5976、EFCC C - 6040、EFCC C - 6849、EFCC C - 7268、EFCC C - 7342、EFCC C - 7992、EFCC C - 8027和EFCC C - 8549）中培育出了体外子实体。从体外培养的子实体中分离出单个子囊孢子，并通过菌株内杂交和杂交用于在糙米培养基中生产子实体。测量了体外生长的子座的长度和干重。选择产生子座干重最高的菌株。发现单个子囊孢子菌株的菌株内杂交和菌株间杂交均能产生大量的蛹虫草子实体。
