Sugita Takashi, Tajima Mami, Takashima Masako, Amaya Misato, Saito Masuyoshi, Tsuboi Ryoji, Nishikawa Akemi
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Microbiol Immunol. 2004;48(8):579-83. doi: 10.1111/j.1348-0421.2004.tb03554.x.
Over the last few years, new Malassezia species have been found regularly in Japanese subjects. We isolated another new Malassezia species from a Japanese patient with seborrheic dermatitis (SD), and named it M. yamatoensis. In its physiological characteristics and the utilization of Tween by M. yamatoensis is similar to that of M. furfur and M. dermatis. It is distinguished by its growth temperature. To examine the distribution of the microorganism in the skin of patients with SD and atopic dermatitis (AD), and healthy subjects, we applied transparent dressings to the skin, and detected M. yamatoensis DNA using a non-culture-based method that consisted of nested PCR with specific primers. M. yamatoensis DNA was detected from 3 of 31 SD patients (9.7%), 5 of 36 AD patients (13.9%), and 1 of 22 healthy subjects (4.6%). Therefore, M. yamatoensis is a rare member of the cutaneous microflora.
在过去几年中，日本人群中经常发现新型马拉色菌。我们从一名患有脂溢性皮炎（SD）的日本患者身上分离出另一种新型马拉色菌，并将其命名为大和马拉色菌。大和马拉色菌的生理特征及对吐温的利用情况与糠秕马拉色菌和皮肤马拉色菌相似。它通过生长温度来区分。为了检测该微生物在SD患者、特应性皮炎（AD）患者及健康受试者皮肤中的分布情况，我们将透明敷料贴于皮肤上，并使用一种基于非培养的方法（由带有特异性引物的巢式PCR组成）检测大和马拉色菌DNA。在31例SD患者中有3例（9.7%）检测到了大和马拉色菌DNA，36例AD患者中有5例（13.9%）检测到，22例健康受试者中有1例（4.6%）检测到。因此，大和马拉色菌是皮肤微生物群中一种罕见的成员。
