Sugita Takashi, Takashima Masako, Kodama Minako, Tsuboi Ryoji, Nishikawa Akemi
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
J Clin Microbiol. 2003 Oct;41(10):4695-9. doi: 10.1128/JCM.41.10.4695-4699.2003.
Lipophilic yeasts of the genus Malassezia are part of the normal cutaneous microflora and are considered one of the factors that trigger atopic dermatitis (AD). We isolated two strains of Malassezia from a healthy Japanese female. Analysis of the D1/D2 26S ribosomal DNA and internal transcribed spacer region sequences of the isolates suggested that they are new members of the genus Malassezia. We propose the name Malassezia japonica sp. nov. for the isolates. M. japonica is easily distinguished from the seven known lipophilic species by its ability to assimilate Tween 40 and Tween 60 and its inability to assimilate Tween 20 and Tween 80 and to grow at 40 degrees C. Furthermore, by applying transparent dressings to the skin lesions of 36 patients with AD and the skin of 22 healthy subjects, M. japonica DNA was detected by a non-culture-based method consisting of nested PCR with M. japonica species-specific primers. M. japonica DNA was detected from 12 of the 36 patients (33.3%) and 3 of the 22 healthy subjects (13.6%). Although it is not known whether M. japonica plays a role in AD, this species was part of the microflora in both patients with AD and healthy subjects.
马拉色菌属的亲脂性酵母是正常皮肤微生物群的一部分，被认为是引发特应性皮炎（AD）的因素之一。我们从一名健康的日本女性身上分离出两株马拉色菌。对分离株的D1/D2 26S核糖体DNA和内部转录间隔区序列的分析表明，它们是马拉色菌属的新成员。我们提议将分离株命名为日本马拉色菌（Malassezia japonica sp. nov.）。日本马拉色菌通过其同化吐温40和吐温60的能力以及不能同化吐温20和吐温80以及在40℃生长的能力，很容易与七种已知的亲脂性物种区分开来。此外，通过对36例AD患者的皮肤病变和22名健康受试者的皮肤应用透明敷料，采用由日本马拉色菌属特异性引物的巢式PCR组成的非培养方法检测到了日本马拉色菌DNA。在36例患者中有12例（33.3%）检测到日本马拉色菌DNA，在22名健康受试者中有3例（13.6%）检测到。虽然尚不清楚日本马拉色菌是否在AD中起作用，但该物种在AD患者和健康受试者中都是微生物群的一部分。
