Osaki Hideki, Nomura Kinya, Matsumoto Naoyuki, Ohtsu Yoshihiro
National Institute of Fruit Tree Science, Fujimoto, Tsukuba, Ibaraki 305-8605, Japan.
Mycol Res. 2004 Jun;108(Pt 6):635-40. doi: 10.1017/s095375620400005x.
Double-stranded (ds) RNA of various types was detected by electrophoresis in 23 of 25 isolates of Helicobasidium mompa. These dsRNAs varied in size from ca. 2 kbp to more than 10 kbp. dsRNAs from an isolate V1 had two distinct nucleotide sequences for putative RNA-dependent RNA polymerase (RDRP). Their complete sequences revealed that V1 dsRNA1 was 2247 bp in length, with a single ORF that encoded a 706-amino acid residue polypeptide with a predicted molecular mass of 82.6 kDa, and that V1 dsRNA3 was 1776 bp in length, with a single ORF that encoded a 538-amino acid residue polypeptide with a predicted molecular mass of 62.6 kDa. RDRP-conserved motifs were identified in both predicted amino acid sequences. Phylogenetic analysis indicated that V1 dsRNA1 was most closely related to Fusarium poae virus 1, while V1 dsRNA3 was most closely related to Helicobasidium mompa 70 virus. These results indicate coinfection of isolate V1 by two distinct partitiviruses.
在25株桑卷担菌分离物中的23株中，通过电泳检测到了各种类型的双链（ds）RNA。这些dsRNA的大小从约2 kbp到超过10 kbp不等。分离物V1的dsRNA具有两种不同的假定RNA依赖性RNA聚合酶（RDRP）核苷酸序列。它们的完整序列显示，V1 dsRNA1长度为2247 bp，有一个单一的开放阅读框，编码一个706个氨基酸残基的多肽，预测分子量为82.6 kDa；V1 dsRNA3长度为1776 bp，有一个单一的开放阅读框，编码一个538个氨基酸残基的多肽，预测分子量为62.6 kDa。在两个预测的氨基酸序列中都鉴定出了RDRP保守基序。系统发育分析表明，V1 dsRNA1与燕麦镰刀菌病毒1关系最为密切，而V1 dsRNA3与桑卷担菌70病毒关系最为密切。这些结果表明分离物V1被两种不同的分体病毒共同感染。
