Huang Weiquan, Sun Lan, Lü Baozhen, Wang Wei, Pu Ruolei, Chen Lei, Xia Yongjuan
Department of Histology and Embryology, the Fourth Military Medical University, Xi'an 710032, People's Republic of China.
J Mol Histol. 2004 Jan;35(1):47-53. doi: 10.1023/b:hijo.0000021063.86024.48.
5-Hydroxytryptamine (5-HT) and its receptor have been localized and quantified in the submaxillary gland of rats of various ages, using immunohistochemistry, in situ hybridization and in situ quantification. In male rats, the epithelial cells of serous acini, intercalated ducts, secretary tubes and excretory ducts all showed 5-HT and 5-HT receptor (5-HTR) immunoreactivity. Both 5-HT and 5-HTR reactive sites were found in the same cells of adjacent sections. 5-HT1A receptor mRNA hybridized signals could be detected in cytoplasm of these cells. The parasympathetic ganglia cells and endothelial cells of small vessels also showed 5-HT and 5-HTR immunoreactivity in the cytoplasm. However, in female rats, only the epithelial cells in excretory tubes showed 5-HT and 5-HTR immunoreactivity. The immunoreactivity was present in the same cells of adjacent sections. The relative content of 5-HT and its receptor increased during the first 60 postnatal days but remained constant from day 60 to day 90 postnatum. These results suggest that the submaxillary gland of rats possess autocrine 5-HT, which may regulate the function and development of the gland.
利用免疫组织化学、原位杂交和原位定量技术，对不同年龄大鼠的颌下腺中5-羟色胺（5-HT）及其受体进行了定位和定量分析。在雄性大鼠中，浆液性腺泡、闰管、分泌管和排泄管的上皮细胞均显示出5-HT和5-HT受体（5-HTR）免疫反应性。在相邻切片的同一细胞中发现了5-HT和5-HTR反应位点。在这些细胞的细胞质中可检测到5-HT1A受体mRNA杂交信号。副交感神经节细胞和小血管内皮细胞的细胞质中也显示出5-HT和5-HTR免疫反应性。然而，在雌性大鼠中，仅排泄管中的上皮细胞显示出5-HT和5-HTR免疫反应性。免疫反应性存在于相邻切片的同一细胞中。5-HT及其受体的相对含量在出生后的前60天增加，但从出生后第60天到第90天保持恒定。这些结果表明，大鼠颌下腺具有自分泌5-HT，可能调节腺体的功能和发育。
