Chu Chenyu, Gao Guodong, Huang Weiquan
Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an City, 710032, China.
J Mol Histol. 2008 Feb;39(1):49-55. doi: 10.1007/s10735-007-9125-2. Epub 2007 Aug 3.
It has been known that GnRH, LH and their receptors exist in hippocampal neurons. However, whether FSH and its receptor also exist in hippocampal neurons remained unknown yet. In situ hybridization, double-labeled immunofluorescence stain and double-labeled immunohistochemistry stain in adjacent sections were used in our research to study the distribution, co-localization of FSH and its receptor and co-localization of FSH and GnRH receptor in rat hippocampus. The result found that pyramidal neurons from CA1 to CA4 region and granule neurons in dentate gyrus could express FSH and its receptor, majority of hippocampal neurons co-expressed FSH and its receptor, FSH and GnRH receptor. These suggested that hippocampal neurons not only express FSH but also act as FSH target cells. FSH may regulate the function of hippocampal neurons by ways of paracrine or autocrine. At the same time, GnRH may regulate the function of FSH neuron in hippocampus through GnRH receptor.
已知促性腺激素释放激素（GnRH）、促黄体生成素（LH）及其受体存在于海马神经元中。然而，卵泡刺激素（FSH）及其受体是否也存在于海马神经元中仍不清楚。本研究采用原位杂交、相邻切片双标免疫荧光染色和双标免疫组化染色，研究FSH及其受体在大鼠海马中的分布、共定位以及FSH与GnRH受体的共定位。结果发现，从CA1到CA4区的锥体细胞和齿状回颗粒细胞可表达FSH及其受体，大多数海马神经元共表达FSH及其受体、FSH和GnRH受体。这些结果提示，海马神经元不仅表达FSH，还作为FSH的靶细胞。FSH可能通过旁分泌或自分泌方式调节海马神经元的功能。同时，GnRH可能通过GnRH受体调节海马中FSH神经元的功能。
