Quílez A M, Saenz M T, García M D, de la Puerta R
Departamento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, C/ Profesor García Gonzalez no. 2, 41012-Seville, Spain.
J Pharm Pharmacol. 2004 Sep;56(9):1185-9. doi: 10.1211/0022357044102.
Agave intermixta Trel. (Maguey) and Cissus sicyoides L. (Bejuco caro) are Caribbean plant species from the Dominican Republic used locally in traditional popular medicine that have shown an antiinflammatory effect in experimental animal models. A phytochemical analysis on these species allowed us the isolation and identification of the steroidal sapogenins hecogenin and diosgenin from Maguey and the hydroxystilbene resveratrol from Bejuco caro. The effects of these plant extracts and their isolated constituents on compound-48/80-induced histamine release from peritoneal mast cells were investigated. Significant inhibition was produced by 0.5 mg mL(-1) of a methanolic extract of Bejuco (41.1%) and by its constituent resveratrol (82.4%) at a dose of 250 microM. However, none of the steroidal sapogenins from A. intermixta showed a significant inhibitory effect on histamine release from mast cells. From these results, it can be deduced that the in-vitro anti-allergic activity towards the release of histamine from mast cells shown by the methanolic extract of C. sicyoides may be mediated by its constituent resveratrol and might contribute to the antiinflammatory activity shown by this species.
混生龙舌兰（特赖尔）（龙舌兰）和西番莲（西番莲科）是来自多米尼加共和国的加勒比植物物种，在当地传统民间医学中使用，已在实验动物模型中显示出抗炎作用。对这些物种进行的植物化学分析使我们能够从龙舌兰中分离和鉴定出甾体皂苷元海柯皂苷元和薯蓣皂苷元，以及从西番莲中分离出羟基芪白藜芦醇。研究了这些植物提取物及其分离成分对化合物48/80诱导的腹膜肥大细胞组胺释放的影响。西番莲甲醇提取物0.5 mg/mL（41.1%）及其成分白藜芦醇在250 μM剂量下（82.4%）产生了显著抑制作用。然而，混生龙舌兰中的甾体皂苷元均未对肥大细胞组胺释放显示出显著抑制作用。从这些结果可以推断，西番莲甲醇提取物对肥大细胞组胺释放的体外抗过敏活性可能由其成分白藜芦醇介导，并且可能有助于该物种显示出的抗炎活性。
