和其茎的成分及其生物活性。
The Constituents of the Stems of and Their Bioactivities.
机构信息
Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan 71005, Taiwan.
Graduate Institute of Natural Products, School of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
出版信息
Molecules. 2018 Oct 28;23(11):2799. doi: 10.3390/molecules23112799.
Fifty-five compounds were isolated from the fresh stems of , including 14 benzenoids, 11 triterpenes, nine steroids, five tocopherols, five chlorophylls, four flavonoids, two benzoquinones, two tannins, and three other compounds. Their structures were constructed by 1D and 2D nuclear magnetic resonance (NMR) and mass spectral data, and were also identified by a comparison of their spectral data with those reported in the literature. Among these isolates, 1,2-bis-(5--tocopheryl) ethane () was reported for the first time from natural sources. Some purified compounds were examined for their anti-inflammatory and anticancer bioactivities. The results indicated that betulinic acid () exhibited strong inhibition of superoxide anion generation with IC value of 0.2 ± 0.1 μM, while betulinic acid () and pheophytin-a () inhibited elastase release with IC value of 2.7 ± 0.3 and 5.3 ± 1.0 μM, respectively. In addition, betulinic acid () and -glut-5(6)-en-ol () exhibited potential cytotoxicity to non-small-cell lung carcinoma (NCI-H226) and colon cancer (HCT-116) cell lines with IC values in the range of 1.6 to 9.1 μM.
从新鲜的茎中分离得到了 55 种化合物，包括 14 种苯丙素、11 种三萜、9 种甾体、5 种生育酚、5 种叶绿素、4 种类黄酮、2 种苯醌、2 种单宁和 3 种其他化合物。通过 1D 和 2D 核磁共振（NMR）和质谱数据构建了它们的结构，并通过将其光谱数据与文献中报道的数据进行比较来鉴定。在这些分离物中，1,2-双-（5--生育酚基）乙烷（）是首次从天然来源中报道的。一些纯化的化合物被检测其抗炎和抗癌的生物活性。结果表明，白桦脂酸（）对超氧阴离子生成的抑制作用较强，IC 值为 0.2±0.1μM，而白桦脂酸（）和脱镁叶绿酸-a（）对弹性蛋白酶释放的抑制作用分别为 2.7±0.3 和 5.3±1.0μM。此外，白桦脂酸（）和-谷氨酰-5(6)-烯醇（）对非小细胞肺癌（NCI-H226）和结肠癌细胞（HCT-116）表现出潜在的细胞毒性，IC 值范围为 1.6 至 9.1μM。
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