Laboratório de Secreção de Mastócitos, Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil.
J Ethnopharmacol. 2011 May 17;135(2):463-8. doi: 10.1016/j.jep.2011.03.046. Epub 2011 Mar 29.
Different plant species from Cordia genera are used in folk medicine as anti-inflammatory medication throughout the tropical and subtropical regions of the world. In Brazil, Cordia verbenacea is a medicinal plant known as "erva-baleeira". The alcoholic extracts, decoctions and infusions with leaves of C. verbenacea are used in Brazilian traditional medicine for treatment of cough, pneumonia, parasitic diseases and, especially, the inflammatory processes. Anti-inflammatory activity was already demonstrated; however, molecular mechanisms of action are not completely understood. Considering the importance of histamine in early events of inflammation and in allergic diseases, we evaluated the effect of ethanol extract of leaves of C. verbenacea on histamine release (in vitro and in vivo studies) from different types of mast cells induced by chemical agents using several species of rodents.
The extraction and quantification of histamine were performed by using an automatic fluorometric continuous flow system.
The extract of C. verbenacea (30 μg/ml) reduced the in vitro secretion of histamine from rat mast cells induced by ionophore A23187, concanavalin A and compound 48/80, respectively, to 22.1 ± 2.2%, 24.3 ± 2.5% and 21.4 ± 2.1%. At the same concentration, the extract also inhibited the secretion of histamine from mast cells of guinea pig induced by ionophore A23187 to 33.3 ± 2.2%, and mast cells of hamster induced by ionophore A23187 and concanavalin A to 15.8 ± 2.5% and 10.8 ± 2.6%, respectively. The oral treatment with the extract (300 mg/kg) also inhibited the secretion of histamine induced by A23187 about to 36.3 ± 3.2% in rats.
C. verbenacea inhibits the in vitro secretion of histamine from mast cells of different animal species, as well as the secretion of mast cells from animals treated with the extract, which gives not only the proven anti-inflammatory effect of the plant, but also anti-allergic effect, opening new possibilities for future anti-allergic herbal medicine.
来自 Cordia 属的不同植物物种在世界热带和亚热带地区的民间医学中被用作抗炎药物。在巴西，Cordia verbenacea 是一种药用植物，被称为“erva-baleeira”。用 C. verbenacea 的叶子制成的酒精提取物、煎剂和浸剂被用于巴西传统医学中，用于治疗咳嗽、肺炎、寄生虫病，特别是炎症过程。抗炎活性已经得到证实；然而，作用机制尚不完全清楚。鉴于组胺在炎症早期事件和过敏疾病中的重要性，我们评估了叶乙醇提取物对不同类型肥大细胞诱导的组胺释放（体外和体内研究）的影响化学试剂使用几种啮齿动物。
使用自动荧光连续流动系统进行组胺的提取和定量。
C. verbenacea 提取物（30 μg/ml）分别将离子载体 A23187、刀豆球蛋白 A 和化合物 48/80 诱导的大鼠肥大细胞体外分泌的组胺减少至 22.1±2.2%、24.3±2.5%和 21.4±2.1%。在相同浓度下，该提取物还抑制了离子载体 A23187 诱导的豚鼠肥大细胞组胺的分泌，抑制率为 33.3±2.2%，离子载体 A23187 和刀豆球蛋白 A 诱导的仓鼠肥大细胞组胺的分泌，抑制率为 15.8±2.5%和 10.8±2.6%。提取物（300mg/kg）的口服治疗也抑制了 A23187 诱导的组胺的分泌，抑制率约为 36.3±3.2%大鼠。
C. verbenacea 抑制了不同动物物种肥大细胞中组胺的体外分泌，以及用提取物处理的动物肥大细胞中组胺的分泌，这不仅证明了植物的抗炎作用，而且具有抗过敏作用，为未来的抗过敏草药开辟了新的可能性。
