Paisano A F, Spira B, Cai S, Bombana A C
Department of Endodontics/University of São Paulo, São Paulo, Brazil.
Oral Microbiol Immunol. 2004 Oct;19(5):327-30. doi: 10.1111/j.1399-302x.2004.00166.x.
This study assessed the effect of bacteriophages on the viability of Enterococcus faecalis. Human dental roots were inoculated with a suspension of E. faecalis at three different multiplicities of infection - 0.1, 1.0 and 10.0. The phage lysate was able to significantly inhibit bacteria growth when incubated at the multiplicities of infection of 1.0, 10.0 and 0.1. The dental roots were also inoculated with bacteria for 6 days to allow bacterial penetration into the teeth tubules. Addition of the phage lysate to the roots following the 6-day incubation period led to a substantial reduction in bacteria viability. Phage therapy may be an important alternative for the treatment of root canal infections refractory to conventional endodontic therapy.
本研究评估了噬菌体对粪肠球菌生存能力的影响。将人牙根管接种三种不同感染复数（0.1、1.0和10.0）的粪肠球菌悬液。当在感染复数为1.0、10.0和0.1下孵育时，噬菌体裂解物能够显著抑制细菌生长。牙根管也接种细菌6天，以使细菌渗入牙小管。在6天孵育期后向根管中添加噬菌体裂解物导致细菌生存能力大幅降低。噬菌体疗法可能是治疗对传统牙髓治疗难治的根管感染的一种重要替代方法。
