Tanriverdi F, Esener T, Erganiş O, Belli S
Department of Conservative Dentistry and Endodontics, Selçuk University, Faculty of Dentistry, Konya, Türkiye.
Braz Dent J. 1997;8(2):67-72.
The aim of the present study was to develop an in vitro test model from human teeth to comparatively examine antibacterial effectiveness of calcium hydroxide, parachlorophenol (PCP) and camphorated parachlorophenol (CPCP) against Enterococcus faecalis in infected root canals. Cylindrical dentin specimens were prepared from freshly extracted human maxillary anterior teeth. The specimens were inoculated with E. faecalis and then medicated with either CPCP, PCP or Ca(OH)2. The disinfecting efficacy of these agents was tested by collecting dentin chips from the inner ("canal") walls of the specimens and counting viable E. faecalis. The dentin chips were diluted and a classical bacterial count technique was used for recovery of E. faecalis strains of 5% sheep blood agar. The effectiveness of CPCP and PCP at one day was superior to the effectiveness of Ca(OH)2. In the three-day group, CPCP was the most effective, followed by Ca(OH)2. The experimental model used in this study may be useful for investigation of the effect of intracanal medicaments on microorganisms lodged in the root dentinal tubules.
本研究的目的是建立一种源自人牙的体外测试模型，以比较氢氧化钙、对氯苯酚（PCP）和樟脑对氯苯酚（CPCP）对感染根管内粪肠球菌的抗菌效果。从新鲜拔除的人上颌前牙制备圆柱形牙本质标本。将标本接种粪肠球菌，然后用CPCP、PCP或Ca(OH)₂进行药物处理。通过从标本的内壁（“根管”）收集牙本质碎屑并对存活的粪肠球菌进行计数，来测试这些药物的消毒效果。将牙本质碎屑稀释，并使用经典的细菌计数技术在5%绵羊血琼脂上复苏粪肠球菌菌株。CPCP和PCP在一天时的效果优于Ca(OH)₂。在三天组中，CPCP最有效，其次是Ca(OH)₂。本研究中使用的实验模型可能有助于研究根管内药物对滞留在牙根牙本质小管中的微生物的作用。
