Moreira-Santos Matilde, Soares Amadeu M V M, Ribeiro Rui
Instituto do Ambiente e Vida, Departamento de Zoologia da Universidade de Coimbra, Largo Marquês de Pombal, 3004-517 Coimbra, Portugal.
Ecotoxicol Environ Saf. 2004 Oct;59(2):164-73. doi: 10.1016/j.ecoenv.2003.07.004.
This study was designed to evaluate the suitability of an in situ microalgal bioassay with Pseudokirchneriella subcapitata to be used in freshwater environments. The assay potential was investigated by deploying it in a system impacted by acid mine drainage. Water samples were collected to perform a laboratory assay also. P. subcapitata was viewed to be a good option for the in situ assay because it grew well and according to control acceptability criteria when immobilized in calcium alginate beads. A reduction in algal growth was apparent at both impacted sites demonstrating assay sensitivity: the site closest to the effluent discharge was clearly impacted and the one further downstream appeared to be moderately impacted. Results from the laboratory assay, designed to distinguish effects of nutrient differences across sites from those due to the effluent, confirmed the in situ responses. Results are discussed in light of the significance of combining information from different assessment tools, namely in situ and laboratory assays, as well as water-quality parameters, particularly at sites that are moderately impacted.
本研究旨在评估用斜生栅藻进行原位微藻生物测定法在淡水环境中的适用性。通过将该测定法部署在受酸性矿山排水影响的系统中来研究其测定潜力。还采集了水样进行实验室测定。斜生栅藻被认为是原位测定的一个良好选择，因为当固定在海藻酸钙珠中时，它生长良好且符合对照可接受标准。在两个受影响的地点，藻类生长均明显减少，表明该测定法具有敏感性：最靠近废水排放口的地点受到明显影响，而更下游的地点似乎受到中度影响。旨在区分不同地点营养差异与废水造成的影响的实验室测定结果证实了原位反应。结合不同评估工具（即原位和实验室测定）以及水质参数所提供信息的重要性进行了讨论，特别是在受到中度影响的地点。
