Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai 264003, China.
Sensors (Basel). 2013 May 24;13(6):6936-45. doi: 10.3390/s130606936.
A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation-allylthiourea and thioacetamide-have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 μM and 0.46 μM for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.
一种使用氨氧化菌（AOB）Nitrosomonas europaea 作为生物受体和聚合物膜铵选择性电极作为传感器的用于检测水中毒性的流动生物传感器。该系统基于毒物对 AOB 活性的抑制作用，可通过用铵选择性膜电极测量铵消耗率来评估。AOB 细胞固定在聚醚砜膜上，填充在支架中，而膜电极放置在流动池的下游。已经测试了两种氨氧化的特定抑制剂-丙烯基硫脲和硫代乙酰胺。定义为导致氨氧化活性降低 50%的抑制剂浓度的 IC50 值分别为 0.17 μM 和 0.46 μM。该传感器具有简单、快速和高灵敏度的优点，可用于测量水中的毒性。
