Matsui Elizabeth C, Krop Esmeralda J M, Diette Gregory B, Aalberse Rob C, Smith Abigail L, Eggleston Peyton A
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Ann Allergy Asthma Immunol. 2004 Aug;93(2):171-8. doi: 10.1016/S1081-1206(10)61471-8.
Although there is evidence that contact with mice is associated with IgE-mediated mouse sensitization and mouse specific antibody responses, the exposure-response relationships remain unclear.
To determine whether IgE-mediated mouse sensitization and mouse specific IgG (mIgG) and mIgG4 levels increase with increasing Mus m 1 exposure.
One hundred fifty-one workers at a mouse research and production facility were studied. Exposure assignments were made by linking participants to airborne Mus m 1 concentrations in their respective work areas. Cumulative exposure was estimated by multiplying airborne Mus m 1 concentration by duration of employment. Serum mIgG and mIgG4 levels were quantified by antigen-binding assays, and IgE-mediated mouse sensitization was evaluated by skin prick testing (SPT).
Prevalence rates of mouse SPT sensitivity and of high levels of mIgG and mIgG4 were increasingly higher by quintiles of increasing cumulative exposure (P < .01 for SPT, mIgG, and mIgG4). After adjusting for age, sex, and atopy, the log odds ratio (OR) of having positive mouse SPT results was linearly related to cumulative exposure (r2 = 0.87), as was the log OR of having a high mIgG level (r2 = 0.86). Quintile of cumulative exposure was an independent predictor of both SPT sensitivity (OR, 1.7; 95% confidence interval, 1.2-2.5) and a high mIgG level (OR, 1.7; 95% confidence interval, 1.2-2.4).
IgE-mediated mouse sensitization and mIgG and mIgG4 levels were related to cumulative exposure in a dose-dependent manner. Thus, strategies to prevent allergy to mice should remain focused on reducing mouse allergen exposure.
尽管有证据表明接触小鼠与IgE介导的小鼠致敏和小鼠特异性抗体反应有关，但暴露-反应关系仍不清楚。
确定IgE介导的小鼠致敏以及小鼠特异性IgG（mIgG）和mIgG4水平是否随Mus m 1暴露增加而升高。
对一家小鼠研究和生产设施中的151名工人进行了研究。通过将参与者与其各自工作区域空气中的Mus m 1浓度相关联来进行暴露分配。累积暴露量通过空气中Mus m 1浓度乘以就业持续时间来估算。血清mIgG和mIgG4水平通过抗原结合试验进行定量，IgE介导的小鼠致敏通过皮肤点刺试验（SPT）进行评估。
随着累积暴露量的增加，小鼠SPT敏感性以及高水平mIgG和mIgG4的患病率在五分位数中越来越高（SPT、mIgG和mIgG4的P < 0.01）。在调整年龄、性别和特应性后，小鼠SPT结果呈阳性的对数优势比（OR）与累积暴露呈线性相关（r2 = 0.87），高mIgG水平的对数OR也是如此（r2 = 0.86）。累积暴露五分位数是SPT敏感性（OR，1.7；95%置信区间，1.2 - 2.5）和高mIgG水平（OR，1.7；95%置信区间，1.2 - 2.4）的独立预测因素。
IgE介导的小鼠致敏以及mIgG和mIgG4水平与累积暴露呈剂量依赖性关系。因此，预防小鼠过敏的策略应继续侧重于减少小鼠过敏原暴露。
