Matsui E C, Diette G B, Krop E J M, Aalberse R C, Smith A L, Eggleston P A
Johns Hopkins University, Baltimore, MD, USA.
Clin Exp Allergy. 2006 Aug;36(8):1097-103. doi: 10.1111/j.1365-2222.2006.02534.x.
High serum levels of cat-specific IgG and IgG4 are associated with protection against allergic sensitization to cat, but whether this association applies to other animal allergens remains unclear.
To determine if high levels of mouse-specific IgG and IgG4 are associated with a decreased risk of mouse skin test sensitivity.
Two hundred and sixty workers of a mouse facility underwent skin prick testing and completed a questionnaire. Serum levels of mouse-specific IgG and IgG4 were quantified by solid-phase antigen binding assays. Room air samples were collected and airborne Mus m 1 was quantified by ELISA.
Forty-nine participants had a positive skin prick test to mouse. Mouse-specific IgG was detected in 219 (84%) participants and IgG4 was detected in 72 (28%) participants. A detectable mouse-specific IgG4 level was associated with an increased risk of mouse skin test sensitivity (odds ratios (OR) 6.4, 95% confidence intervals (CI) 3.3-12.4). Mouse-specific IgG and IgG4 were both positively correlated with mouse allergen exposure (r(s)=0.31, P=0.0001, and r(s)=0.27, P=0.0006, respectively). The odds of skin test sensitivity peaked at moderate levels of IgG4, but decreased at the highest levels of mouse-specific IgG4. In contrast, the odds of skin test sensitivity increased monotonically with IgG levels.
A detectable level of mouse-specific IgG4 is associated with an increased risk of skin test sensitivity to mouse. However, the highest IgG4 levels appear to be associated with an attenuated risk of mouse skin test sensitivity, suggesting that induction of high levels of IgG4 through natural exposure may protect against the development of allergic sensitization.
血清中高水平的猫特异性IgG和IgG4与预防对猫的过敏致敏有关，但这种关联是否适用于其他动物过敏原仍不清楚。
确定高水平的小鼠特异性IgG和IgG4是否与小鼠皮肤试验敏感性降低风险相关。
对一家小鼠饲养设施的260名工作人员进行皮肤点刺试验并完成问卷调查。通过固相抗原结合试验定量测定血清中小鼠特异性IgG和IgG4的水平。收集室内空气样本，通过ELISA定量测定空气中的小家鼠Mus m 1。
49名参与者对小鼠皮肤点刺试验呈阳性。219名（84%）参与者检测到小鼠特异性IgG，72名（28%）参与者检测到IgG4。可检测到的小鼠特异性IgG4水平与小鼠皮肤试验敏感性增加风险相关（优势比（OR）6.4，95%置信区间（CI）3.3 - 12.4）。小鼠特异性IgG和IgG4均与小鼠过敏原暴露呈正相关（分别为r(s)=0.31，P = 0.0001和r(s)=0.27，P = 0.0006）。皮肤试验敏感性的几率在IgG4中等水平时达到峰值，但在小鼠特异性IgG4最高水平时降低。相比之下，皮肤试验敏感性的几率随IgG水平单调增加。
可检测到的小鼠特异性IgG4水平与对小鼠皮肤试验敏感性增加风险相关。然而，最高的IgG4水平似乎与小鼠皮肤试验敏感性风险降低相关，这表明通过自然暴露诱导高水平的IgG4可能预防过敏致敏的发生。
