优化从全血中提取RNA用于微阵列基因表达分析的产量。
Optimizing RNA extraction yield from whole blood for microarray gene expression analysis.
作者信息
Wang Jian, Robinson John F, Khan Hafiz M R, Carter David E, McKinney James, Miskie Brooke A, Hegele Robert A
机构信息
Vascular Biology Research Group and London Regional Genomics Centre, Robarts Research Institute and University of Western Ontario, London, ON, N6A 5K8, Canada.
出版信息
Clin Biochem. 2004 Sep;37(9):741-4. doi: 10.1016/j.clinbiochem.2004.03.013.
OBJECTIVES
Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications.
DESIGN AND METHODS
We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip.
CONCLUSIONS
Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
目的
对血液白细胞的基因表达谱进行微阵列分析有许多潜在的临床和研究应用。
设计与方法
我们使用PAXgene血液RNA系统，通过两种孵育时间从正常志愿者的全血中制备RNA，随后使用Affymetrix HU133A基因芯片进行基因表达谱分析。
结论
较长的孵育时间可显著提高RNA产量，获得的样本足以用于微阵列分析，且重复样本间具有出色的成对相关性。
Zotero 插件