Liang Ke-shan, Peng Li-jing, Yin Cheng-bin, Zhang Jing-ling, Xu Cong-gao, Liu Xiang-dong, Du Jing-yun, Chen Wei-ning
The People's Hospital of Pingyi County, Shandong Province 273300, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004 Jun;18(2):165-7.
To observe cytopathogenic effect of Hantaan virus (HV) on cultured human bone marrow cells.
Light and transmission electron microscopy and direct immunofluorescent technique were applied to study cellular structure especially ultrastructural changes of bone marrow cells from patients with Hantaan virus infection. Bone marrow cells of one healthy volunteer were also studied as control.
The antigen of HV was found in bone marrow cells of 20 of 27 HFRS patients by the aid of direct immunofluorescent technique. It was found that the granulocytes had the highest percentage of HV antigen positive cells (76%), followed by monocytes (65%), lymphocytes (40%), megakaryocytes (20%) and the lowest was found in erythrocytes (3.7%). The injury of cell membrane after infection with HV was significantly more severe than that in the control group under the light and electron microscopy.
This study demonstrated that HV could attack human bone marrow cells and cause cytopathogenic effect on them.
观察汉坦病毒（HV）对培养的人骨髓细胞的细胞病变效应。
应用光镜、透射电镜及直接免疫荧光技术研究汉坦病毒感染患者骨髓细胞的细胞结构，尤其是超微结构变化。同时以1名健康志愿者的骨髓细胞作为对照进行研究。
借助直接免疫荧光技术在27例肾综合征出血热患者中的20例骨髓细胞中发现了HV抗原。发现粒细胞中HV抗原阳性细胞的比例最高（76%），其次是单核细胞（65%）、淋巴细胞（40%）、巨核细胞（20%），红细胞中最低（3.7%）。光镜和电镜下观察发现，感染HV后细胞膜的损伤明显比对照组严重。
本研究表明，HV可侵袭人骨髓细胞并对其产生细胞病变效应。
