[人巨细胞病毒感染致病因素的初步研究]
[Preliminary studies on pathogenic factors of human cytomegalovirus infection].
作者信息
Li Ai-bin, Liu Qian, Xia Liang-bin, Li Yu-hong
机构信息
The Ren Min Hospital of Wu Han University, Wuhan 430060, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Dec;17(4):369-71.
OBJECTIVE
To investigate the pathogenic factors of human cytomegalovirus (HCMV) infections.
METHODS
Totally 36 serum samples were obtained from early pregnant woman and examined with ELISA for anti-HCMV antibody IgG and IgM. After artificial abortion,chorionic villus and decidua were also examined with polymerase chain reaction (PCR) for HCMV-DNA. When the results of PCR were positive, pathological changes of these chorionic villus and decidua were analyzed.
RESULTS
The results showed that only 10 samples were PCR positive while IgG and/or IgM antibody to HCMV was positive. After infection with HCMV, different changes occurred in chorionic villus and decidual trophoblastic cells placental villus were hyperplasic and decidua cells degenerated and necrotized followed by lymphocytes infiltration.
CONCLUSION
These pathological changes may be one of pathogenic factors of HCMV.
目的
探讨人巨细胞病毒（HCMV）感染的致病因素。
方法
收集36例早孕妇女血清样本，采用酶联免疫吸附测定法（ELISA）检测抗HCMV抗体IgG和IgM。人工流产后，采用聚合酶链反应（PCR）检测绒毛膜和蜕膜组织中的HCMV-DNA。PCR结果为阳性时，分析这些绒毛膜和蜕膜组织的病理变化。
结果
结果显示，仅10份样本的PCR结果呈阳性，同时伴有HCMV的IgG和/或IgM抗体阳性。感染HCMV后，绒毛膜和蜕膜滋养层细胞出现不同变化，胎盘绒毛增生，蜕膜细胞变性坏死，随后有淋巴细胞浸润。
结论
这些病理变化可能是HCMV致病因素之一。
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