[广东省SARS病毒的分离与鉴定]

[Isolation and identification of SARS virus in Guangdong province].

作者信息

Yan Xin-ge, Wan Zhou-yue, Zhang Xin, Zheng Qiu-xia, Zheng Kui, Huang Ji-cheng, Huang Ping, Lu Jia-hai

机构信息

Center for Diseases Control and Prevention of Guangdong province, Guangzhou 510300, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):213-6.

PMID:15340560

Abstract

BACKGROUND

To isolate and identify pathogen of atypical pneumonia in Guangdong.

METHODS

Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.

RESULTS

A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR. And sequence assay showed that only 39-65 percent of sequence of the virus was homogenous to known coronavirus, but almost 100% homogenous (with one base exception, 12a to t) to SARS-associated coronavirus isolated from patients outside Guangdong, such as in Beijing, Hong Kong, Taiwan, Germany, Italy and so on. Indirect immunofluorescence test showed a specific antigen-antibody reactivity between the coronavirus and convalescent-phase sera of SARS patients.

CONCLUSION

The pathogen of the atypical pneumonia in Guangdong province was a novel type of coronavirus, which could be isolated by using MDCK cells.

摘要

背景

分离并鉴定广东省非典型肺炎的病原体。

方法

采用MDCK细胞从非典型肺炎患者采集的多种样本中分离病原体，并采用血清学和分子方法进行鉴定。

结果

从非典型肺炎患者中分离出一种新型冠状病毒，通过巢式RT-PCR扩增出一段279 nt的RNA片段。序列分析表明，该病毒与已知冠状病毒的序列同源性仅为39% - 65%，但与从广东以外地区患者（如北京、香港、台湾、德国、意大利等）分离出的SARS相关冠状病毒几乎100%同源（有一个碱基例外，12a突变为t）。间接免疫荧光试验显示该冠状病毒与SARS患者恢复期血清之间存在特异性抗原 - 抗体反应。