Park Y, Katzir N, Brotman Y, King J, Bertrand F, Havey M
Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA.
Theor Appl Genet. 2004 Aug;109(4):707-12. doi: 10.1007/s00122-004-1684-y. Epub 2004 May 5.
Zucchini yellow mosaic virus (ZYMV) routinely causes significant losses in cucumber ( Cucumis sativus L.) and melon ( Cucumis melo L.). ZYMV resistances from the cucumber population 'TMG1' and the melon plant introduction (PI) 414723 show different modes of inheritance and their genetic relationships are unknown. We used molecular markers tightly linked to ZYMV resistances from cucumber and melon for comparative mapping. A 5-kb genomic region (YCZ-5) cosegregating with the zym locus of cucumber was cloned and sequenced to reveal single nucleotide polymorphisms and indels distinguishing alleles from ZYMV-resistant (TMG1) and susceptible (Straight 8) cucumbers. A low-copy region of the YCZ-5 clone was hybridized to bacterial artificial chromosome (BAC) clones of melon and a 180-kb contig assembled. One end of this melon contig was mapped in cucumber and cosegregated with ZYMV resistance, demonstrating that physically linked regions in melon show genetic linkage in cucumber. However the YCZ-5 region segregated independently of ZYMV resistance loci in two melon families. These results establish that these sources of ZYMV resistances from cucumber TMG1 and melon PI414723 are likely non-syntenic.
西葫芦黄花叶病毒（ZYMV）经常在黄瓜（Cucumis sativus L.）和甜瓜（Cucumis melo L.）上造成重大损失。黄瓜群体“TMG1”和甜瓜种质资源引进品种（PI）414723中的ZYMV抗性表现出不同的遗传模式，且它们的遗传关系未知。我们使用与黄瓜和甜瓜的ZYMV抗性紧密连锁的分子标记进行比较作图。克隆并测序了一个与黄瓜zym基因座共分离的5kb基因组区域（YCZ-5），以揭示区分ZYMV抗性（TMG1）和易感（Straight 8）黄瓜等位基因的单核苷酸多态性和插入缺失。将YCZ-5克隆的一个低拷贝区域与甜瓜的细菌人工染色体（BAC）克隆杂交，并组装成一个180kb的重叠群。该甜瓜重叠群的一端在黄瓜中定位并与ZYMV抗性共分离，表明甜瓜中物理连锁的区域在黄瓜中显示出遗传连锁。然而，在两个甜瓜家族中，YCZ-5区域与ZYMV抗性位点独立分离。这些结果表明，黄瓜TMG1和甜瓜PI414723的这些ZYMV抗性来源可能是非共线的。
