Park Y H, Sensoy S, Wye C, Antonise R, Peleman J, Havey M J
Department of Horticulture, University of Wisconsin, Madison 53706, USA.
Genome. 2000 Dec;43(6):1003-10.
The watermelon strain of papaya ringspot virus (PRSV-W) and zucchini yellow mosaic virus (ZYMV) are potyviruses that cause significant disease losses in cucumber. Resistances have been identified primarily in exotic germplasm that require transfer to elite cultivated backgrounds. To select more efficiently for virus resistances, we identified molecular markers tightly linked to PRSV-W and ZYMV resistances in cucumber. We generated F6 recombinant inbred lines (RILs) from a cross between Cucumis sativus L. 'Straight 8' and a line from 'Taichung Mou Gua', TMG1 (susceptible and resistant, respectively, to both viruses), and studied the segregations of amplified fragment length polymorphism (AFLP) markers, randomly amplified polymorphic DNAs (RAPDs), restriction fragment length polymorphisms (RFLPs), and resistances to PRSV-W and ZYMV. A 353-point map of cucumber was generated, delineating 12 linkage groups at LOD 3.5. Linkage arrangements among RFLPs were consistent with previously published maps; however linkages among RAPDs in our map did not agree with a previously published map. Resistances to PRSV-W and ZYMV were tightly linked (2.2 cM) and mapped to the end of one linkage group. One AFLP cosegregated with resistance to ZYMV.
番木瓜环斑病毒西瓜株系（PRSV-W）和西葫芦黄花叶病毒（ZYMV）是引起黄瓜重大病害损失的马铃薯Y病毒属病毒。抗性主要在需要导入优良栽培背景的外来种质中被鉴定出来。为了更有效地筛选病毒抗性，我们在黄瓜中鉴定了与PRSV-W和ZYMV抗性紧密连锁的分子标记。我们从黄瓜‘Straight 8’与‘台中木瓜’品系TMG1（分别对两种病毒敏感和抗性）的杂交后代中产生了F6重组自交系（RILs），并研究了扩增片段长度多态性（AFLP）标记、随机扩增多态性DNA（RAPD）、限制性片段长度多态性（RFLP）的分离情况以及对PRSV-W和ZYMV的抗性。构建了一个包含353个位点的黄瓜图谱，在LOD值为3.5时划定了12个连锁群。RFLP之间的连锁排列与先前发表的图谱一致；然而，我们图谱中RAPD之间的连锁与先前发表的图谱不一致。对PRSV-W和ZYMV的抗性紧密连锁（2.2 cM），并定位在一个连锁群的末端。一个AFLP标记与对ZYMV的抗性共分离。
